Invitrogen

GLO1 Monoclonal Antibody (Glo1a)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

6 References

View all (29) GLO1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GLO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

GLO1 Antibody (MA1-13029) in IHC (P)

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human Prostate and Stomach cancer tissues. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed i... View More

GLO1 Antibody in Immunohistochemistry (IHC)

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GLO1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of GLO1 was observed in Mouse Liver and Rat Liver in comparison to Mouse Skeletal Muscle using Anti-GLO1 Monoclonal Antibody (Product # MA1-13029X) in Western Blot. {RE}

Product Details

MA1-13029

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:800

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

Glo1a

Immunogen

Recombinant human GLOI-GST fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154644

Target Information

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulating Morphological and Redox/Glycative Alterations in the PCOS Uterus: Effects of Carnitines in PCOS Mice.

Biomedicines

Palmerini MG,Macchiarelli G,Cocciolone D,Mascitti IA,Placidi M,Vergara T,Di Emidio G,Tatone C

MA1-13029 was used in Western Blotting to investigate morphological and molecular alterations in the PCOS uterus and search for modulating effects of different carnitine formulations.

Fri Jan 27 00:00:00 EST 2023

Modulating Morphological and Redox/Glycative Alterations in the PCOS Uterus: Effects of Carnitines in PCOS Mice.

Biomedicines

Palmerini MG,Macchiarelli G,Cocciolone D,Mascitti IA,Placidi M,Vergara T,Di Emidio G,Tatone C

MA1-13029 was used in Western Blotting to investigate morphological and molecular alterations in the PCOS uterus and search for modulating effects of different carnitine formulations.

Fri Jan 27 00:00:00 EST 2023

Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.

Cells

Emidio GD,Placidi M,Rea F,Rossi G,Falone S,Cristiano L,Nottola S,D'Alessandro AM,Amicarelli F,Palmerini MG,Tatone C

Published figure using GLO1 monoclonal antibody (Product # MA1-13029) in Western Blot

Tue Jan 14 00:00:00 EST 2020

Glycine Suppresses AGE/RAGE Signaling Pathway and Subsequent Oxidative Stress by Restoring Glo1 Function in the Aorta of Diabetic Rats and in HUVECs.

Oxidative medicine and cellular longevity

Wang Z,Zhang J,Chen L,Li J,Zhang H,Guo X

MA1-13029 was used in Western Blotting to evaluate whether glycine can attenuate oxidative stress by suppressing the AGE/RAGE signalling pathway in the aorta of streptozotocin-induced diabetic rats and in human umbilical vascular endothelial cells.

Tue Apr 23 00:00:00 EDT 2019

Inhibition of Glyoxalase-I Leads to Reduced Proliferation, Migration and Colony Formation, and Enhanced Susceptibility to Sorafenib in Hepatocellular Carcinoma.

Frontiers in oncology

Michel M,Hollenbach M,Pohl S,Ripoll C,Zipprich A

MA1-13029 was used in Immunohistochemistry to analyse Glo-I in hepatocellular carcinoma and the effect of the multi-tyrosine kinase inhibitor sorafenib on Glo-I.

Thu Oct 01 00:00:00 EDT 2020

Glycine increases glyoxalase-1 function by promoting nuclear factor erythroid 2-related factor 2 translocation into the nucleus of kidney cells of streptozotocin-induced diabetic rats.

Journal of diabetes investigation

Wang Z,Zhao D,Chen L,Li J,Yuan G,Yang G,Zhang H,Guo X,Zhang J

MA1-13029 was used in Western Blotting to determine the role of glyoxalase-1 and activation of the nuclear factor erythroid 2-related factor 2 in the mitigation of oxidative stress in rat kidneys.

Sun Sep 01 00:00:00 EDT 2019

Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.

Nature medicine

Qi W,Keenan HA,Li Q,Ishikado A,Kannt A,Sadowski T,Yorek MA,Wu IH,Lockhart S,Coppey LJ,Pfenninger A,Liew CW,Qiang G,Burkart AM,Hastings S,Pober D,Cahill C,Niewczas MA,Israelsen WJ,Tinsley L,Stillman IE,Amenta PS,Feener EP,Vander Heiden MG,Stanton RC,King GL

MA113029 was used in western blot to study the role of pyruvate kinase M2 in diabetic nephropathy

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Aldoketomutase; epididymis secretory protein Li 74; Glx I; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural; glyoxalase domain containing 1; Glyoxalase I; glyoxylase 1; Ketone-aldehyde mutase; lactoyl glutathione lyase; Lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

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Gene Aliases: 0610009E22Rik; 1110008E19Rik; 2510049H23Rik; AW550643; Glo-1; Glo-1r; Glo-1s; GLO1; Glo1-r; Glo1-s; GLOD1; GLY1; GLYI; HEL-S-74; Qglo

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UniProt ID: (Human) Q04760, (Rat) Q6P7Q4, (Mouse) Q9CPU0

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Entrez Gene ID: (Human) 2739, (Rat) 294320, (Mouse) 109801

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Function(s)

lactoylglutathione lyase activity zinc ion binding lyase activity metal ion binding lyase

Process(es)

carbohydrate metabolic process pyruvate metabolic process regulation of transcription from RNA polymerase II promoter glutathione metabolic process methylglyoxal metabolic process osteoclast differentiation negative regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GLO1 Monoclonal Antibody (Glo1a)

GLO1 Antibody (MA1-13029) in IHC (P)

Product Details

MA1-13029

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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