Proteintech

GRB2 Polyclonal Antibody

GRB2 Antibody in Immunocytochemistry (ICC/IF)

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GRB2 Antibody (10254-2-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 10254-2-AP (GRB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GRB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

10254-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GRB2 Fusion Protein Ag0395 (1-214 aa encoded by BC000631)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEAIAKYDF KATADDELSF KRGDILKVLN EECDQNWYKA ELNGKDGFIP KNYIEMKPHP WFFGKIPRAK AEEMLSKQRH DGAFLIRESE SAPGDFSLSV KFGNDVQHFK VLRDGAGKYF LWVVKFNSLN ELVDYHRSTS VSRNQQIFLR DIEQVPQQPT YVQALFDFDP QEDGELGFRR GDFIHVMDNS DPNWWKGACH GQTGMFPRNY VTPVN (1-214 aa encoded by BC000631)

Target Information

GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

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Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2.

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Oncotarget

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mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.

Oncotarget

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mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.

Oncotarget

Wang X,Lu X,Zhang T,Wen C,Shi M,Tang X,Chen H,Peng C,Li H,Fang Y,Deng X,Shen B

Tue Apr 19 00:00:00 EDT 2016

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Oncotarget

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Bioinformatics

Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2

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Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2

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UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994

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Entrez Gene ID: (Human) 2885, (Mouse) 14784, (Rat) 81504

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Function(s)

SH3/SH2 adaptor activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding neurotrophin TRKA receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH3 domain binding protein kinase binding protein phosphatase binding identical protein binding insulin receptor substrate binding poly(A) RNA binding ephrin receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity phosphotyrosine binding protein domain specific binding phosphoprotein binding scaffold/adaptor protein

Process(es)

MAPK cascade epidermal growth factor receptor signaling pathway Ras protein signal transduction cell-cell signaling axon guidance insulin receptor signaling pathway fibroblast growth factor receptor signaling pathway positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling viral process positive regulation of actin filament polymerization T cell costimulation receptor internalization phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway signal transduction in response to DNA damage regulation of MAPK cascade positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling anatomical structure formation involved in morphogenesis leukocyte migration branching involved in labyrinthine layer morphogenesis cellular response to ionizing radiation positive regulation of reactive oxygen species metabolic process aging cell differentiation protein heterooligomerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRB2 Polyclonal Antibody

GRB2 Antibody (10254-2-AP) in ICC/IF

Product Details

10254-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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