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GRB2 Antibody (PA5-141994) in WB
Western Blot analysis of GRB2 in MOLT4 (A) and Human Thymus (B) lysates (35 µg protein in RIPA buffer) using GRB2 Polyclonal Antibody, Biotin (Product # PA5-141994) at 0.3 µg/mL incubated for 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Product Details
PA5-141994
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-PRNYVTPVNRNV.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932657
Product Specific Information
Additional Information: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2
Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2
UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994
Entrez Gene ID: (Human) 2885, (Pig) 100192436, (Mouse) 14784, (Rat) 81504
SH3/SH2 adaptor activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH3 domain binding
protein kinase binding
protein phosphatase binding
identical protein binding
insulin receptor substrate binding
poly(A) RNA binding
ephrin receptor binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
phosphotyrosine binding
protein domain specific binding
phosphoprotein binding
scaffold/adaptor protein
MAPK cascade
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
cell-cell signaling
axon guidance
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
positive regulation of signal transduction
regulation of phosphatidylinositol 3-kinase signaling
viral process
positive regulation of actin filament polymerization
T cell costimulation
receptor internalization
phosphatidylinositol-3-phosphate biosynthetic process
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB2 signaling pathway
negative regulation of epidermal growth factor receptor signaling pathway
signal transduction in response to DNA damage
regulation of MAPK cascade
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
anatomical structure formation involved in morphogenesis
leukocyte migration
branching involved in labyrinthine layer morphogenesis
cellular response to ionizing radiation
positive regulation of reactive oxygen species metabolic process
aging
cell differentiation
protein heterooligomerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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