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GTF2H3 Antibody (CF812723) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2H3 (RC209399, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2H. Positive lysates LY419886 (100 µg) and LC419886 (20 µg) can be purchased separately from OriGene.
Product Details
CF812723
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4B5
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-241 of human GTF2H3 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
GTF2H3 interacts with HIV-1 Tat as a component of the HIV-1 transcription pre-initiation complex, but is released from the elongation complex which includes P-TEFb. It synergizes with HIV-1 Tat to induce transcription elongation from the HIV-1 LTR promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic transcription factor 2 34 kDa subunit; BTF2 p34; BTF2-p34; General transcription factor IIH polypeptide 3; General transcription factor IIH subunit 3; general transcription factor IIH, polypeptide 3, 34kDa; TFIIH basal transcription factor complex p34 subunit
Gene Aliases: 34kDa; 5033417D07Rik; BTF2; BTF2 p34; C730029A10; D5Ertd679e; GTF2H3; P34; TFB4; TFIIH
UniProt ID: (Human) Q13889, (Mouse) Q8VD76, (Rat) Q561R7
Entrez Gene ID: (Human) 2967, (Mouse) 209357, (Rat) 288651
damaged DNA binding
transcription factor activity, sequence-specific DNA binding
protein kinase activity
protein binding
DNA-dependent ATPase activity
translation factor activity, RNA binding
RNA polymerase II carboxy-terminal domain kinase activity
metal ion binding
protein N-terminus binding
molecular_function
general transcription factor
RNA metabolism protein
nucleic acid metabolism protein
nucleotide-excision repair, DNA duplex unwinding
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
translation
nucleotide-excision repair, DNA incision
phosphorylation of RNA polymerase II C-terminal domain
global genome nucleotide-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
protein phosphorylation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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