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Invitrogen
F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, PE, eBioscience™
FIGURE: 1 / 7
Mouse IgG (H+L) Secondary Antibody (12-4010-82) in Flow
Staining of a mixture of normal human iPSC and the murine C2C12 cell line with Anti-Human TRA-1-60 (Podocalyxin) PerCP-eFluor® 710 (Product # 46-8863-82) and 0.25 µg of Mouse IgG2a kappa Isotype Control, Purified (Product # 14-4724-82) (left) or 0.25 µg of Anti-Human NLGN4X (Neuroligin-4) Purified (right) followed by F(ab’)2-Goat anti-Mouse IgG PE (Product # 12-4010-82). Viable cells, as determined by Fixab... View More
Product Details
12-4010-82
Applications
Tested Dilution
Flow Cytometry (Flow)
0.25 µg/test
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11063706
Target
IgG
Antibody Form
F(ab')2 Fragment
Product Specific Information
Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.
This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).
Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.
Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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