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Invitrogen
Goat anti-Rat IgG (H+L) Secondary Antibody, TRITC, eBioscience™
FIGURE: 1 / 6
Rat IgG (H+L) Secondary Antibody (26-4826-82) in ICC/IF
Immunocytochemistry on fixed and permeabilized C2C12 cells using 5 µg/mL of Rat IgG2a Isotype Control (left) or 5 µg/mL of Anti-Mouse/Rat Ki-67 Purified (right) followed by Anti-Rat TRITC. Nuclei are stained with DAPI. Co-expression of Ki-67 and DAPI appears pink.
Product Details
26-4826-82
Applications
Tested Dilution
Immunohistochemistry (Frozen) (IHC (F))
Assay-Dependent
Immunocytochemistry (ICC/IF)
5 µg/mL
Flow Cytometry (Flow)
1 µg/test
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
552/578 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469694
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Description: This goat anti-rat IgG polyclonal antibody reacts with rat IgG and is useful for indirect immunofluorescent staining for flow cytometric analysis.
Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.
Applications Tested: This polyclonal antibody has been tested by flow cytometric detection of rat primary antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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