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Invitrogen
Goat anti-Rat IgG (H+L) Secondary Antibody, Rhodamine
FIGURE: 1 / 1
Rat IgG (H+L) Secondary Antibody (31680) in ICC/IF
Immunofluorescence analysis of Goat anti-Rat IgG (H+L) Secondary Antibody, Rhodamine conjugate was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Rat primary antibody for 3 hou... View More
Product Details
31680
Applications
Tested Dilution
Immunohistochemistry (IHC)
1:50 - 1:200
Immunocytochemistry (ICC/IF)
4 µg/mL
Flow Cytometry (Flow)
1:50 - 1:200
Immunoprecipitation (IP)
1:50 - 1:200
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
573/591 nm
View spectra
Form
Lyophilized
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228357
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31680 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31680 reacts with the heavy chains of rat IgG and with the light chains common to most rat immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, antibodies may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.47 (lot-dependent).
Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration).
Country of Origin: USA
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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