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H2AK9ac Antibody (PA5-101008) in ICC/IF
Immunocytochemistry analysis of H2AK9ac in Hela cells (heat shock treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with H2AK9ac polyclonal antibody (Product # PA5-101008) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101008
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human Histone H2A around the acetylation site of Lys9.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815848
Product Specific Information
Antibody detects endogenous levels of Acetyl-Histone H2A only when acetylated at Lys9.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyl-Histone H2; Acetyl-Histone H2A K9; Acetyl-Histone H2A Lys9; H2A 615; H2A GL101; H2A histone family member; H2A histone family, member A; H2A histone family, member C; H2A histone family, member E; H2A histone family, member G; H2A histone family, member M; H2A histone family, member P; H2A histone family, member R; H2A histone family, member X; H2A histone family, member Z; H2A-clustered histone 1; H2A-clustered histone 12; H2A-clustered histone 6; H2A.1; H2A/d; H2a/x; H2A/z; h2ad; H2ae; h2ai; H2ak; H2AK5ac; H2ALys9ac; H2AX histone; H2AZ histone; H2B histone family, member U, (testis-specific); HIS; HIST3H2A; histone 1, H2aa; histone 1, H2ab; histone 1, H2ad; histone 1, H2ae; histone 1, H2ag; histone 1, H2ah; histone 1, H2ai; histone 1, H2aj; histone 1, H2ba; histone 2, H2ai; histone 5 protein 2ax; Histone H2A type 1; Histone H2A type 1-A; Histone H2A type 1-B; Histone H2A type 1-B/E; Histone H2A type 1-C; Histone H2A type 1-D; Histone H2A type 1-E; Histone H2A type 1-G; Histone H2A type 1-H; Histone H2A type 1-I; Histone H2A type 1-J; Histone H2A type 1-N; Histone H2A type 1-O; Histone H2A type 1-P; Histone H2A type 4; Histone H2A, testis; Histone H2A.2; Histone H2A.3; Histone H2A.X; Histone H2A.Z; Histone H2A/a; Histone H2A/e; Histone H2A/g; Histone H2A/m; histone H2A/p; Histone H2A/ptl; Histone H2A/r; Histone H2A/s; histone H2AD; histone H2AE; Histone H2AX; Histone H2B type 1-A; Histone H2B, testis; MGC74460; similar to Histone H2A type 1; testis-specific histone 2a; testis-specific histone 2b; Testis-specific histone H2B; TH2A; TSH2B.1
Gene Aliases: 1190022L06; AW228881; bA317E16.2; bA317E16.3; dJ160A22.4; dJ86C11.1; gammaH2ax; H2a-291A; H2a-53; H2A.1; H2A.1b; H2A.2; H2A.3; H2A.X; H2A.Z; H2A.Z-1; H2A/a; H2A/c; H2A/E; H2A/g; H2A/m; H2A/p; H2A/S; H2A/X; H2A/z; H2AA; H2AC1; H2AC11; H2AC12; H2AC13; H2AC14; H2AC15; H2AC16; H2AC17; H2AC4; H2ac6; H2AC7; H2AC8; H2AFA; H2AFALii; H2AFC; H2AFD; H2AFE; H2AFG; H2AFI; H2AFM; H2AFN; H2AFP; H2AFR; H2AFX; H2AFZ; H2AG; H2AH; H2AX; H2AZ; H2AZ1; H2BC1; H2BFU; HIST1H2AA; HIST1H2AB; Hist1h2ac; HIST1H2AD; HIST1H2AE; HIST1H2AG; HIST1H2AH; HIST1H2AI; HIST1H2AJ; HIST1H2AK; HIST1H2AL; HIST1H2AM; Hist1h2an; Hist1h2ao; Hist1h2ap; HIST1H2BA; Hist5-2ax; pH2A/f; RGD1566119; STBP; TH2A; TH2B; TSH2B; TSH2B.1
UniProt ID: (Human) P16104, (Human) P0C0S5, (Mouse) P27661, (Mouse) P0C0S6, (Rat) P0C0S7, (Human) Q96QV6, (Human) P04908, (Human) P20671, (Human) P0C0S8, (Human) Q96KK5, (Human) Q99878, (Human) Q96A08, (Rat) Q00728, (Mouse) C0HKE6, (Mouse) Q8CGP6, (Rat) Q00729
Entrez Gene ID: (Human) 3014, (Human) 3015, (Rat) 500987, (Mouse) 15270, (Mouse) 51788, (Rat) 58940, (Human) 221613, (Human) 8335, (Human) 3013, (Human) 3012, (Human) 8969, (Human) 85235, (Human) 8329, (Human) 8331, (Human) 255626, (Rat) 24828, (Mouse) 319172, (Mouse) 319165, (Mouse) 319166, (Mouse) 319167, (Mouse) 319168, (Mouse) 319191, (Rat) 24829, (Rat) 680322
DNA binding
damaged DNA binding
protein binding
enzyme binding
histone binding
protein heterodimerization activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin DNA binding
nucleosomal DNA binding
molecular_function
histone
DNA damage checkpoint
double-strand break repair via homologous recombination
double-strand break repair
double-strand break repair via nonhomologous end joining
nucleosome assembly
chromatin silencing
cellular response to DNA damage stimulus
spermatogenesis
response to ionizing radiation
viral process
cerebral cortex development
positive regulation of DNA repair
meiotic cell cycle
cellular response to gamma radiation
cellular senescence
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
DNA repair
DNA recombination
cell cycle
multicellular organism development
cellular response to insulin stimulus
negative regulation of cell proliferation
biological_process
nucleosome disassembly
inflammatory response
plasminogen activation
spermatogenesis, exchange of chromosomal proteins
positive regulation of binding
mononuclear cell migration
chromatin organization
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