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FIGURE: 1 / 1
HAMP Antibody (PA5-90884) in WB
Western blot analysis of HAMP in extracts of MDA-MB-435 cells. Samples were incubated with HAMP Polyclonal antibody (Product # PA5-90884) followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-90884
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human Hepcidin (NP_0669981)
Conjugate
Form
Liquid
Concentration
4.11 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806273
Product Specific Information
Immunogen sequence: MALSSQIWAA CLLLLLLLAS LTSGSVFPQQ TGQLAELQPQ DRAGARASWM PMFQRRRRRD THFPICIFCC GCCHRSKCGM CCKT; Positive Samples: MDA-MB-435
Target Information
HAMP is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hepcidin; hepcidin antimicrobial peptide 1; LEAP-1; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor
Gene Aliases: HAMP; Hamp1; HEPC; Hepc1; HFE2B; LEAP1; PLTR; UNQ487/PRO1003
UniProt ID: (Human) P81172, (Mouse) Q9EQ21
Entrez Gene ID: (Human) 57817, (Mouse) 84506
negative regulation of transcription from RNA polymerase II promoter
cellular iron ion homeostasis
immune response
response to iron ion
killing of cells of other organism
defense response to bacterium
defense response to fungus
multicellular organismal iron ion homeostasis
negative regulation of intestinal absorption
myeloid cell homeostasis
JAK-STAT cascade
negative regulation of iron ion transmembrane transport
positive regulation of macrophage activation
negative regulation of bone resorption
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
iron ion homeostasis
positive regulation of cellular protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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