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HAND1 Antibody (PA5-97267) in WB
Western blot analysis of HAND1 in extracts of various cell lines. Samples were incubated with HAND1 Polyclonal antibody (Product # PA5-97267) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-97267
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 16-215 of human HAND1 (NP_0048121)
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809069
Product Specific Information
Immunogen sequence: PHPAHPMLHE PFLFGPASRC HQERPYFQSW LLSPADAAPD FPAGGPPPAA AAAATAYGPD ARPGQSPGRL EALGGRLGRR KGSGPKKERR RTESINSAFA ELRECIPNVP ADTKLSKIKT LRLATSYIAY LMDVLAKDAQ SGDPEAFKAE LKKADGGRES KRKRELQQHE GFPPALGPVE KRIKGRTGWP QQVWALELNQ; Positive Samples: SH-SY5Y, A-549, NIH/3T3; Cellular Location: Nucleus, nucleolus, nucleoplasm
Target Information
HAND1 belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHa27; Class A basic helix-loop-helix protein 27; eHAND; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; Heart- and neural crest derivatives-expressed protein 1; Helix-loop-helix transcription factor expressed in extraembryonic mesoderm and trophoblast; Th1; Thing-1
Gene Aliases: BHLHA27; EHAND; Ehand1; HAND1; Hxt; Th1; Thing1
UniProt ID: (Human) O96004, (Mouse) Q64279
Entrez Gene ID: (Human) 9421, (Mouse) 15110
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator activity
transcription corepressor activity
protein binding
transcription factor binding
enzyme binding
identical protein binding
protein homodimerization activity
bHLH transcription factor binding
transcription regulatory region DNA binding
protein heterodimerization activity
DNA binding
sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
mesoderm formation
blastocyst development
trophectodermal cell differentiation
heart looping
embryonic heart tube formation
cardiac left ventricle formation
cardiac right ventricle formation
transcription from RNA polymerase II promoter
heart development
embryonic heart tube development
odontogenesis of dentin-containing tooth
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
ventricular cardiac muscle tissue morphogenesis
cardiac septum morphogenesis
mesenchyme development
cartilage morphogenesis
trophoblast giant cell differentiation
negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding
in utero embryonic development
cell fate determination
heart morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
positive regulation of transcription, DNA-templated
determination of heart left/right asymmetry
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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