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HARS Antibody (PA5-106468) in ICC/IF
Immunofluorescent analysis of HARS in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with HARS polyclonal antibody (Product # PA5-106468) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-106468
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HARS(Accession P12081), corresponding to amino acid residues K472-C509.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854137
Product Specific Information
Antibody detects endogenous levels of total HARS.
Target Information
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dead end homolog 1; FLJ20491; HisRS; histidine translase; histidine tRNA ligase; Histidine--tRNA ligase, cytoplasmic; Histidyl-tRNA synthetase; histidyl-tRNA synthetase, cytoplasmic
Gene Aliases: CMT2W; Dnd1; HARS; HARS1; HRS; MMHRS; USH3B
UniProt ID: (Human) P12081, (Mouse) Q61035
Entrez Gene ID: (Human) 3035, (Mouse) 15115, (Rat) 307492
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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