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HBB Antibody (PA5-76877) in WB
Western blot was performed using Anti-HBB Polyclonal Antibody (Product # PA5-76877) and 14 kDa band corresponding to HBB was observed in Rat Liver. Tissue extract (30 µg lysate) of Rat Liver (Lane 1) was electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by Goat Anti-Rabbit IgG Secondary Antibody,... View More
Product Details
PA5-76877
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human HBB.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2720604
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon-gamma-G-gamma-A-delta-beta-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta globin chain; Beta-1-globin; Beta-globin; hemoglobin beta; Hemoglobin beta chain; hemoglobin beta chain complex; Hemoglobin beta chain, major-form; Hemoglobin beta-1 chain; Hemoglobin subunit beta; Hemoglobin subunit beta-1; hemoglobin, beta; III beta-1 globin; III beta-2 globin
Gene Aliases: beta-globin; CD113t-C; HBB
UniProt ID: (Human) P68871, (Rat) P02091
Entrez Gene ID: (Human) 3043, (Rat) 24440
glutathione metabolic process
regulation of eIF2 alpha phosphorylation by heme
oxygen transport
hemopoiesis
erythrocyte development
protein heterooligomerization
receptor-mediated endocytosis
blood coagulation
regulation of blood pressure
positive regulation of cell death
bicarbonate transport
nitric oxide transport
response to hydrogen peroxide
hydrogen peroxide catabolic process
positive regulation of nitric oxide biosynthetic process
regulation of blood vessel size
renal absorption
platelet aggregation
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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