Proteintech

HINT1 Polyclonal Antibody

HINT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HINT1 Antibody (10717-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HINT1 Antibody in Immunoprecipitation (IP)

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Product Details

10717-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:300-1:1200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HINT1 Fusion Protein Ag1142 (1-126 aa encoded by BC007090)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to HINT1.

Immunogen sequence: MADEIAKAQ VARPGGDTIF GKIIRKEIPA KIIFEDDRCL AFHDISPQAP THFLVIPKKH ISQISVAEDD DESLLGHLMI VGKKCAADLG LNKGYRMVVN EGSDGGQSVY HVHLHVLGGR QMHWPPG (1-126 aa encoded by BC007090)

Target Information

Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest.

Brain sciences

Zhang Q,Zhang C,Liu C,Zhan H,Li B,Lu Y,Wei H,Cheng J,Li S,Wang C,Hu C,Liao X

Fri Jul 15 00:00:00 EDT 2022

Comparative Proteomic Analysis of HPV(+) Oropharyngeal Squamous Cell Carcinoma Recurrence.

Journal of proteome research

Ho AS,Robinson A,Shon W,Laury A,Raedschelders K,Venkatraman V,Holewinski R,Zhang Y,Shiao SL,Chen MM,Mallen-St Clair J,Lin DC,Zumsteg ZS,Van Eyk JE

Fri Jan 07 00:00:00 EST 2022

Methods to separate nuclear soluble fractions reflecting localizations in living cells.

iScience

Ogawa Y,Imamoto N

Fri Dec 17 00:00:00 EST 2021

Methods to separate nuclear soluble fractions reflecting localizations in living cells.

iScience

Ogawa Y,Imamoto N

Fri Dec 17 00:00:00 EST 2021

A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide.

Journal of medicinal chemistry

Passow KT,Caldwell HS,Ngo KA,Arnold JJ,Antczak NM,Narayanan A,Jose J,Sturla SJ,Cameron CE,Ciota AT,Harki DA

Thu Oct 28 00:00:00 EDT 2021

The role of the Hint1 protein in the metabolism of phosphorothioate oligonucleotides drugs and prodrugs, and the release of H₂S under cellular conditions.

Biochemical pharmacology

Krakowiak A,Piotrzkowska D,Kocoń-Rębowska B,Kaczmarek R,Maciaszek A

Wed May 01 00:00:00 EDT 2019

Second messenger Ap₄A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.

Nature communications

Yu J,Liu Z,Liang Y,Luo F,Zhang J,Tian C,Motzik A,Zheng M,Kang J,Zhong G,Liu C,Fang P,Guo M,Razin E,Wang J

Fri Oct 11 00:00:00 EDT 2019

Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.

Oncogene

Motzik A,Amir E,Erlich T,Wang J,Kim BG,Han JM,Kim JH,Nechushtan H,Guo M,Razin E,Tshori S

Thu Aug 17 00:00:00 EDT 2017

Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.

Oncogene

Motzik A,Amir E,Erlich T,Wang J,Kim BG,Han JM,Kim JH,Nechushtan H,Guo M,Razin E,Tshori S

Thu Aug 17 00:00:00 EDT 2017

Interactions of cellular histidine triad nucleotide binding protein 1 with nucleosides 5'-O-monophosphorothioate and their derivatives - Implication for desulfuration process in the cell.

Biochimica et biophysica acta

Krakowiak A,Pawłowska R,Kocoń-Rębowska B,Dolot R,Stec WJ

Mon Dec 01 00:00:00 EST 2014

Interactions of cellular histidine triad nucleotide binding protein 1 with nucleosides 5'-O-monophosphorothioate and their derivatives - Implication for desulfuration process in the cell.

Biochimica et biophysica acta

Krakowiak A,Pawłowska R,Kocoń-Rębowska B,Dolot R,Stec WJ

Mon Dec 01 00:00:00 EST 2014

The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.

Cell cycle (Georgetown, Tex.)

Genovese G,Ghosh P,Li H,Rettino A,Sioletic S,Cittadini A,Sgambato A

Fri Jun 01 00:00:00 EDT 2012

The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.

Cell cycle (Georgetown, Tex.)

Genovese G,Ghosh P,Li H,Rettino A,Sioletic S,Cittadini A,Sgambato A

Fri Jun 01 00:00:00 EDT 2012

Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.

International journal of oncology

Huang H,Wei X,Su X,Qiao F,Xu Z,Gu D,Fan H,Chen J

Wed Jun 01 00:00:00 EDT 2011

Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.

International journal of oncology

Huang H,Wei X,Su X,Qiao F,Xu Z,Gu D,Fan H,Chen J

Wed Jun 01 00:00:00 EDT 2011

Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.

International journal of oncology

Huang H,Wei X,Su X,Qiao F,Xu Z,Gu D,Fan H,Chen J

Wed Jun 01 00:00:00 EDT 2011

Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.

International journal of oncology

Huang H,Wei X,Su X,Qiao F,Xu Z,Gu D,Fan H,Chen J

Wed Jun 01 00:00:00 EDT 2011

Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.

The pharmacogenomics journal

Jackson KJ,Chen Q,Chen J,Aggen SH,Kendler KS,Chen X

Mon Aug 01 00:00:00 EDT 2011

Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.

The pharmacogenomics journal

Jackson KJ,Chen Q,Chen J,Aggen SH,Kendler KS,Chen X

Mon Aug 01 00:00:00 EDT 2011

Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.

The pharmacogenomics journal

Jackson KJ,Chen Q,Chen J,Aggen SH,Kendler KS,Chen X

Mon Aug 01 00:00:00 EDT 2011

Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.

The Journal of biological chemistry

Murakami E,Tolstykh T,Bao H,Niu C,Steuer HM,Bao D,Chang W,Espiritu C,Bansal S,Lam AM,Otto MJ,Sofia MJ,Furman PA

Fri Nov 05 00:00:00 EDT 2010

Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.

The Journal of biological chemistry

Murakami E,Tolstykh T,Bao H,Niu C,Steuer HM,Bao D,Chang W,Espiritu C,Bansal S,Lam AM,Otto MJ,Sofia MJ,Furman PA

Fri Nov 05 00:00:00 EDT 2010

Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.

Cancer letters

Zhang YJ,Li H,Wu HC,Shen J,Wang L,Yu MW,Lee PH,Bernard Weinstein I,Santella RM

Wed Mar 18 00:00:00 EDT 2009

Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.

Cancer letters

Zhang YJ,Li H,Wu HC,Shen J,Wang L,Yu MW,Lee PH,Bernard Weinstein I,Santella RM

Wed Mar 18 00:00:00 EDT 2009

Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.

Cancer letters

Zhang YJ,Li H,Wu HC,Shen J,Wang L,Yu MW,Lee PH,Bernard Weinstein I,Santella RM

Wed Mar 18 00:00:00 EDT 2009

Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.

Cancer letters

Zhang YJ,Li H,Wu HC,Shen J,Wang L,Yu MW,Lee PH,Bernard Weinstein I,Santella RM

Wed Mar 18 00:00:00 EDT 2009

Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.

Proteomics

Berglund SR,Santana AR,Li D,Rice RH,Rocke DM,Goldberg Z

Wed Apr 01 00:00:00 EDT 2009

Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.

Proteomics

Berglund SR,Santana AR,Li D,Rice RH,Rocke DM,Goldberg Z

Wed Apr 01 00:00:00 EDT 2009

Novel breast cancer metastasis-associated proteins.

Journal of proteome research

Ho J,Kong JW,Choong LY,Loh MC,Toy W,Chong PK,Wong CH,Wong CY,Shah N,Lim YP

Sun Feb 01 00:00:00 EST 2009

Novel breast cancer metastasis-associated proteins.

Journal of proteome research

Ho J,Kong JW,Choong LY,Loh MC,Toy W,Chong PK,Wong CH,Wong CY,Shah N,Lim YP

Sun Feb 01 00:00:00 EST 2009

Novel breast cancer metastasis-associated proteins.

Journal of proteome research

Ho J,Kong JW,Choong LY,Loh MC,Toy W,Chong PK,Wong CH,Wong CY,Shah N,Lim YP

Sun Feb 01 00:00:00 EST 2009

Novel breast cancer metastasis-associated proteins.

Journal of proteome research

Ho J,Kong JW,Choong LY,Loh MC,Toy W,Chong PK,Wong CH,Wong CY,Shah N,Lim YP

Sun Feb 01 00:00:00 EST 2009

Identifying apoptosis-evasion proteins/pathways in human hepatoma cells via induction of cellular hormesis by UV irradiation.

Journal of proteome research

Hsieh SY,Hsu CY,He JR,Liu CL,Lo SJ,Chen YC,Huang HY

Sat Aug 01 00:00:00 EDT 2009

Identifying apoptosis-evasion proteins/pathways in human hepatoma cells via induction of cellular hormesis by UV irradiation.

Journal of proteome research

Hsieh SY,Hsu CY,He JR,Liu CL,Lo SJ,Chen YC,Huang HY

Sat Aug 01 00:00:00 EDT 2009

Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.

The Journal of biological chemistry

Cen B,Li H,Weinstein IB

Fri Feb 20 00:00:00 EST 2009

Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.

The Journal of biological chemistry

Cen B,Li H,Weinstein IB

Fri Feb 20 00:00:00 EST 2009

Distribution and expression of protein kinase C interactive protein (PKCI/HINT1) in mouse central nervous system (CNS).

Neurochemical research

Liu Q,Puche AC,Wang JB

Tue Jul 01 00:00:00 EDT 2008

Distribution and expression of protein kinase C interactive protein (PKCI/HINT1) in mouse central nervous system (CNS).

Neurochemical research

Liu Q,Puche AC,Wang JB

Tue Jul 01 00:00:00 EDT 2008

Distribution and expression of protein kinase C interactive protein (PKCI/HINT1) in mouse central nervous system (CNS).

Neurochemical research

Liu Q,Puche AC,Wang JB

Tue Jul 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: 17 kDa inhibitor of protein kinase C; adenosine 5'-monophosphoramidase; Adenosine 5'-monophosphoramidase HINT1; Desumoylating isopeptidase HINT1; FLJ30414; FLJ32340; Histidine triad nucleotide-binding protein 1; PKC inhibitor/ interacting protein; PKCI-1; Protein kinase C inhibitor 1; protein kinase C, iota; Protein kinase C-interacting protein 1

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Gene Aliases: AA673479; HINT; HINT1; Ipk1; NMAN; Pkci; PKCI-1; PKCI1; Prkci; PRKCNH1

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UniProt ID: (Human) P49773, (Mouse) P70349, (Rat) P62959

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Entrez Gene ID: (Human) 3094, (Mouse) 15254, (Rat) 690660

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Function(s)

nucleotide binding protein kinase C binding hydrolase activity catalytic activity GPI-anchor transamidase activity coenzyme F390-A hydrolase activity coenzyme F390-G hydrolase activity nucleotide phosphatase

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated signal transduction purine ribonucleotide catabolic process positive regulation of calcium-mediated signaling intrinsic apoptotic signaling pathway by p53 class mediator apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HINT1 Polyclonal Antibody

HINT1 Antibody (10717-1-AP) in IHC (P)

Product Details

10717-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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