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HIRA Antibody (39557) in WB
HIRA mAb (Clone WC119.2H11) tested by Western blot. 5 µg of HIRA mAb (Clone WC15) was used to immunoprecipitate HIRA from 500 µg of colcemid-treated U2OS nuclear extract (lane 2). 5 µg of control mouse IgG was also used (lane 1). The immunoprecipitated protein was detected by Western blotting using HIRA mAb (Clone WC119.2H11) at a dilution of 5 µg/ ml.
Product Details
39557
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
WC119.2H11
Immunogen
This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 8, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2793256
Target Information
HIRA is a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this protein in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochromatin. It plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate protein in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DiGeorge critical region gene 1; HIR histone cell cycle regulation defective homolog A; Protein HIRA; TUP1-like enhancer of split gene 1; TUP1-like enhancer of split protein 1
Gene Aliases: AA138857; D16Ertd95e; DGCR1; Gm15797; HIR; HIRA; N28177; OTTMUSG00000026264; TUP1; TUPLE1
UniProt ID: (Human) P54198, (Mouse) Q61666
Entrez Gene ID: (Human) 7290, (Mouse) 15260
osteoblast differentiation
DNA replication-independent nucleosome assembly
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
gastrulation
anatomical structure morphogenesis
chromatin modification
covalent chromatin modification
muscle cell differentiation
negative regulation of nucleic acid-templated transcription
regulation of transcription, DNA-templated
Performance Guarantee
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