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HMG20B/BRAF35 Antibody (A301-097A) in WB
Detection of human HMG20B/BRAF35 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HMG20B/BRAF35 antibody A301-097A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. HMG20B/BRAF35 was also immunoprecipitated by rabbit anti-HMG20B/BRAF35 antibody BL4545, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes ... View More
Product Details
A301-097A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 25 and 75 of human high-mobility group 20B (BRCA2-associated factor 35).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRCA2-associated factor 35; FLJ26127; high-mobility group 20B; HMG box domain containing 2; HMG box-containing protein 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; SMARCE1-related protein; Sox-like transcriptional factor; Structural DNA-binding protein BRAF35; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1
Gene Aliases: B-raf; B-RAF1; BRAF; BRAF1; BRAF25; BRAF35; HMG20B; HMGX2; HMGXB2; NS7; PP7706; pp8857; RAFB1; SMARCE1R; SOXL
UniProt ID: (Human) P15056, (Human) Q9Y491
Entrez Gene ID: (Human) 673, (Human) 10362
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
small GTPase binding
mitogen-activated protein kinase kinase binding
identical protein binding
protein heterodimerization activity
DNA binding
histone deacetylase activity
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
myeloid progenitor cell differentiation
protein phosphorylation
visual learning
organ morphogenesis
positive regulation of gene expression
negative regulation of fibroblast migration
glucose transport
thyroid gland development
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
cellular response to drug
regulation of cell proliferation
negative regulation of apoptotic process
CD4-positive, alpha-beta T cell differentiation
positive T cell selection
response to peptide hormone
negative regulation of neuron apoptotic process
thymus development
positive regulation of axon regeneration
positive regulation of axonogenesis
protein heterooligomerization
positive regulation of stress fiber assembly
response to cAMP
long-term synaptic potentiation
head morphogenesis
face development
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
establishment of protein localization to membrane
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
transcription, DNA-templated
regulation of transcription, DNA-templated
cell cycle
blood coagulation
histone deacetylation
negative regulation of protein sumoylation
skeletal muscle cell differentiation
positive regulation of neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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