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Product Details
PA5-36085
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 1-50 of human HYD
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2553363
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 309 kDa.
Purity is >95% by SDS-PAGE.
Target Information
EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CH D1S3362; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; ECGF1; EDG-1; EDG1; extraembryonic development; hHYD; Hyperplastic discs protein homolog; Progestin-induced protein; S1P1; S1PR1
Gene Aliases: DD5; EDD; EDD1; HYD; KIAA0896; Rat100; UBR5
UniProt ID: (Human) O95071, (Mouse) Q80TP3
Entrez Gene ID: (Human) 51366, (Rat) 117060, (Mouse) 70790
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin-ubiquitin ligase activity
ubiquitin binding
protein domain specific binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
cell proliferation
positive regulation of protein import into nucleus, translocation
positive regulation of catenin import into nucleus
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
progesterone receptor signaling pathway
positive regulation of canonical Wnt signaling pathway
negative regulation of histone H2A K63-linked ubiquitination
negative regulation of double-strand break repair
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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