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FIGURE: 1 / 1
HYD Antibody (PA5-89459) in WB
Western blot analysis of HYD in extracts of various cell lines. Samples were incubated with HYD Polyclonal antibody (Product # PA5-89459) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Product Details
PA5-89459
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human UBR5 (NP_0569862)
Conjugate
Form
Liquid
Concentration
1.92 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805582
Product Specific Information
Immunogen sequence: SISAGIPKVG VLMESVWNMN DSCRFQLRSP ESLKNMEKAS KTTEAKPESK QEPVKTEMGP PPSPASTCSD ASSIASSASM PYKRRRSTPA PKEEEKVNEE QWSLREVVFV EDVKNVPVGK VLKVDGAYVA VKFPGTSSNT NCQNSSGPDA DPSSLLQDCR LLRIDELQVV KTGGTPKVPD CFQRTPKKLC IPEKTEILAV NVDSKGVHAV L; Positive Samples: U-87MG, 293T, Jurkat, HeLa; Cellular Location: Nucleus
Target Information
EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CH D1S3362; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; ECGF1; EDG-1; EDG1; hHYD; Hyperplastic discs protein homolog; Progestin-induced protein; S1P1; S1PR1
Gene Aliases: DD5; EDD; EDD1; HYD; KIAA0896; UBR5
UniProt ID: (Human) O95071
Entrez Gene ID: (Human) 51366
protein polyubiquitination
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
cell proliferation
positive regulation of protein import into nucleus, translocation
positive regulation of catenin import into nucleus
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
progesterone receptor signaling pathway
positive regulation of canonical Wnt signaling pathway
negative regulation of histone H2A K63-linked ubiquitination
negative regulation of double-strand break repair
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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