Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
Histone H3.3 Antibody (PA5-23288) in WB
Western blot analysis was performed on acid extracts (30 µg lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), A549 (Lane 3), T-47D (Lane 4), HEL 92.1.7 (Lane 5), SW480 (Lane 6), HCT 116 (Lane 7), LNCaP (Lane 8), COS-7 (Lane 9), and K-562 (Lane 10). The blots were probed with Rabbit Anti-Histone H3.3 Polyclonal Antibody (Product # PA5-23288, 0.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band correspon... View More
Product Details
PA5-23288
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Fruit fly,
Horse,
Human,
Marsupial,
Mouse,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 100-150 of human Histone H3.3
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2540812
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CG5825-PA; CG5825-PC; CG5825-PD; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3.4; H3.A; H3.B; H3B; H3FT; H3L-like histone; H3t; His3.3A-PA; His3.3A-PC; His3.3A-PD; histone ; Histone cluster 3; Histone H3.3; histone H3.3 ; histone H3.3-like protein; Histone H3.3A; Histone H3.3B; MGC126886; MGC126888
Gene Aliases: CG5825; CG8989; dH3.3A; Dmel\CG5825; Dmel_CG5825; H3-3A; H3-3B; H3.3; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; His-3.3A; His3.3; His3.3A; His3.3B; PH3; PP781
UniProt ID: (Bovine) Q5E9F8, (Human) P84243, (Fruit fly) C0HL66
Entrez Gene ID: (Bovine) 326601, (Horse) 100054462, (Bovine) 617051, (Human) 3020, (Human) 3021, (Fruit fly) 33736
chromatin silencing at rDNA
nucleosome assembly
DNA replication-independent nucleosome assembly
brain development
blood coagulation
response to hormone
positive regulation of cell growth
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
male meiosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support