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Invitrogen
IDH1 Recombinant Rabbit Monoclonal Antibody (10H39L6)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
IDH1 Antibody (702714) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), U-2OS (Lane 3), MCF-7 (Lane 4) and C2C12 (Lane 5). The blot was probed with Anti-IDH1 Recombinant Rabbit Monoclonal Antibody (Product # 702714, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~46 kDa band corresponding to IDH1 was observed across the cell lines tested.
Product Details
702714
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H39L6
Immunogen
Protein corresponding to human IDH1 [aa2-aa414]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2723257
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Dog, Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; IDH; IDP; IDPc; isocitrate dehydrogenase 1 (NADP+); isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; OTTHUMP00000206464; OTTHUMP00000206465; OTTHUMP00000206466; Oxalosuccinate decarboxylase
Gene Aliases: AI314845; AI788952; E030024J03Rik; HEL-216; HEL-S-26; Id-1; IDCD; IDH; Idh-1; IDH1; IDP; IDPC; PICD
UniProt ID: (Human) O75874, (Mouse) O88844
Entrez Gene ID: (Human) 3417, (Mouse) 15926
magnesium ion binding
isocitrate dehydrogenase (NADP+) activity
receptor binding
protein homodimerization activity
NADP binding
NAD binding
(R)-2-hydroxyglutarate dehydrogenase activity
cadherin binding involved in cell-cell adhesion
oxidoreductase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
metal ion binding
dehydrogenase
glyoxylate cycle
tricarboxylic acid cycle
isocitrate metabolic process
2-oxoglutarate metabolic process
NADPH regeneration
glutathione metabolic process
response to oxidative stress
female gonad development
response to steroid hormone
regulation of phospholipid catabolic process
regulation of phospholipid biosynthetic process
cell-cell adhesion
response to organic cyclic compound
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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