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FIGURE: 1 / 3
IKK epsilon Antibody (PA5-28264) in ICC/IF
IKBKE antibody [N2C3] detects IKBKE protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IKBKE protein stained by IKBKE antibody [N2C3] (Product # PA5-28264) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-28264
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 123 and 636 of Human IKBKE
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545740
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji.
Predicted reactivity: Mouse (84%), Rat (85%), Bovine (88%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IKBKE is an activator of nuclear factor-kappa-B. Over expression of IKBKE has been observed in breast cancer cells, and suppression of IKBKE induces cell death. NF-kappa-B is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IKK-i; IKK-related kinase epsilon; Inducible I kappa-B kinase; inducible IKappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; RP11-534L20.1
Gene Aliases: AW558201; IKBKE; IKK-E; IKK-i; IKKE; IKKepsilon; IKKI; KIAA0151
UniProt ID: (Human) Q14164, (Mouse) Q9R0T8
Entrez Gene ID: (Human) 9641, (Mouse) 56489
protein kinase activity
NF-kappaB-inducing kinase activity
protein binding
ATP binding
IkappaB kinase activity
ubiquitin protein ligase binding
K48-linked polyubiquitin binding
K63-linked polyubiquitin binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
protein phosphorylation
immune response
I-kappaB phosphorylation
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of type I interferon production
response to type I interferon
response to interferon-beta
TRIF-dependent toll-like receptor signaling pathway
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
protein homooligomerization
cellular response to virus
cellular response to DNA damage stimulus
phosphorylation
signal transduction by protein phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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