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IL2RG Antibody (PA5-119907) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of SH-SY5Y cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4 8451. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was... View More
Product Details
PA5-119907
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human IL-2RG aa 301-350
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913479
Product Specific Information
Positive Control: Mouse spleen tissue lysates, SH-SY5Y, SiHa, human spleen tissue, human tonsil tissue.
Subcellular Location: Cell membrane, Cell surface.
Target Information
IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD132; CD132 antigen; common cytokine receptor gamma chain; Cytokine receptor common subunit gamma; gammaC; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL2R gamma; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; p64
Gene Aliases: [g]c; CD132; CIDX; gamma(c); gc; IL-2RG; IL2RG; IMD4; P64; SCIDX; SCIDX1
UniProt ID: (Human) P31785, (Mouse) P34902
Entrez Gene ID: (Human) 3561, (Mouse) 16186
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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