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FIGURE: 1 / 6
IRS1 Antibody (PA1-1058) in WB
Cell biochemistry and function 2021 -
3 FIGURE Over-expression of Smad4 reduced the insulin sensitivity in a cellular model of human insulin resistance. A, The validation of over-expressed Smad4 by western-blot along with relative quantification. B, The glucose consumption was determined by commercial kit. C-G, The expression levels of p-IRbeta Tyr1361 , IRbeta, p-IRS-1 Ser307 , p-IRS-1 Tyr612 , IRS-1, Akt and p-Akt Ser473 were determined by we... View More
Product Details
PA1-1058
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues L(602) H T D D G Y M P M S P G V(615) of human IRS-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325027
Product Specific Information
PA1-1058 detects a IRS-1 in mouse and human cells.
PA1-1058 has been successfully used in Western blot procedures. By Western blot this antibody detects an ~170 kDa protein representing IRS-1 in 3T3 L1 cell extract.
The PA1-1058 immunogen is a synthetic peptide corresponding to residues L(602) H T D D G Y M P M S P G V(615) of human IRS-1. The immunizing peptide is 92% conserved in rats, and mice. This peptide (Cat. # PEP-192) is available for use in neutralization and control experiments.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1
UniProt ID: (Human) P35568, (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Mouse) 16367
signal transducer activity
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
protein kinase binding
protein domain specific binding
MAPK cascade
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of glucose metabolic process
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
phosphatidylinositol-3-phosphate biosynthetic process
glucose homeostasis
response to peptide hormone
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
phosphatidylinositol phosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of glucose import in response to insulin stimulus
positive regulation of mesenchymal cell proliferation
regulation of gene expression
lipid catabolic process
insulin secretion
positive regulation of cell migration
mammary gland development
response to caffeine
protein localization to nucleus
positive regulation of phosphorylation
protein kinase B signaling
protein heterooligomerization
positive regulation of glucagon secretion
negative regulation of somatostatin secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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