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IRS2 Antibody (A301-433A) in WB
Detection of human IRS2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody A301-433A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies A301-432A and A301-434A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B... View More
Product Details
A301-433A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1100 and 1150 of human insulin receptor substrate 2.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Insulin receptor substrate 2; IRS-2
Gene Aliases: IRS-2; IRS2
UniProt ID: (Human) Q9Y4H2
Entrez Gene ID: (Human) 8660
signal transducer activity
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
protein kinase binding
protein phosphatase binding
protein domain specific binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
14-3-3 protein binding
MAPK cascade
positive regulation of mesenchymal cell proliferation
negative regulation of B cell apoptotic process
glucose metabolic process
signal transduction
brain development
cell proliferation
positive regulation of cell proliferation
insulin receptor signaling pathway
response to glucose
negative regulation of plasma membrane long-chain fatty acid transport
positive regulation of glucose metabolic process
regulation of phosphatidylinositol 3-kinase signaling
regulation of lipid metabolic process
positive regulation of cell migration
mammary gland development
positive regulation of B cell proliferation
positive regulation of fatty acid beta-oxidation
positive regulation of insulin secretion
cellular response to insulin stimulus
negative regulation of kinase activity
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of GTPase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
lipid homeostasis
cellular response to glucose stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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