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IkB beta Antibody (PA5-79737) in ICC/IF
Immunofluorescence analysis of IKB beta was performed using A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IKB beta Rabbit Polyclonal Antibody (Product # PA5-79737) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution... View More
Product Details
PA5-79737
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human IKB beta recombinant protein (Position: E56-E237).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746852
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunits of the kinase and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I kappa B beta; I-kappa-B-beta; ikappaBbeta; IKB B; IKB beta; IkB-B; ikB-beta; IkBbeta; NF kappa BIB; NF-kappa-B inhibitor beta; NF-kappa-BIB; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; Thyroid receptor; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP 9; TRIP-9
Gene Aliases: I(Kappa)B(beta); I-kappa-B-beta; IKappaBbeta; IkB; ikB-B; IKB-beta; IKBB; NF-kappa-BIB; NFKBIB; TRIP9
UniProt ID: (Human) Q15653, (Mouse) Q60778
Entrez Gene ID: (Human) 4793, (Rat) 81525, (Mouse) 18036
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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