Invitrogen

Insulin Polyclonal Antibody

9 Published Figures

27 References

View all (74) Insulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

Insulin Antibody (PA1-26938) in IHC (P)

Immunohistochemistry (Paraffin) analysis of Insulin was performed in paraffin-embedded human pancreas tissue using Insulin Polyclonal Antibody (Product # PA1-26938) at a dilution of 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Insulin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Insulin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA1-26938

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage buffer

PBS/whole serum, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_794668

Product Specific Information

This antibody stains beta(b) cells of the Langerhan's islets in human pancreas. IHC Note: prolonged fixation in buffered formalin can destroy the epitope.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Insulin Derived Fibrils Induce Cytotoxicity in vitro and Trigger Inflammation in Murine Models.

Journal of diabetes science and technology

Lewis BE,Mulka A,Mao L,Sharafieh R,Qiao Y,Kesserwan S,Wu R,Kreutzer D,Klueh U

PA1-26938 was used in Immunohistochemistry to characterize in vitro and in vivo toxicity, as well as pro-inflammatory activity of insulin fibrils.

Sun Jan 01 00:00:00 EST 2023

Evidence of islet CADM1-mediated immune cell interactions during human type 1 diabetes.

JCI insight

Sona C,Yeh YT,Patsalos A,Halasz L,Yan X,Kononenko NL,Nagy L,Poy MN

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunohistochemistry

Tue Mar 22 00:00:00 EDT 2022

Evidence of islet CADM1-mediated immune cell interactions during human type 1 diabetes.

JCI insight

Sona C,Yeh YT,Patsalos A,Halasz L,Yan X,Kononenko NL,Nagy L,Poy MN

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunohistochemistry

Tue Mar 22 00:00:00 EDT 2022

Ildr1 gene deletion protects against diet-induced obesity and hyperglycemia.

PloS one

Chandra R,Aryal DK,Douros JD,Shahid R,Davis SJ,Campbell JE,Ilkayeya O,White PJ,Rodriguez R,Newgard CB,Wetsel WC,Liddle RA

PA1-26938 was used in Immunohistochemistry-immunofluorescence to examine the effects of ILDR1 on food intake and metabolic activity using mice with genetically-deleted Ildr1.

Tue Jun 28 00:00:00 EDT 2022

A pharmacological approach assessing the role of mast cells in insulin infusion site inflammation.

Drug delivery and translational research

Kesserwan S,Mao L,Sharafieh R,Kreutzer DL,Klueh U

PA1-26938 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to extend the longevity of insulin infusion sets by mitigating the inflammatory response.

Fri Jul 01 00:00:00 EDT 2022

The IGFBP3/TMEM219 pathway regulates beta cell homeostasis.

Nature communications

D'Addio F,Maestroni A,Assi E,Ben Nasr M,Amabile G,Usuelli V,Loretelli C,Bertuzzi F,Antonioli B,Cardarelli F,El Essawy B,Solini A,Gerling IC,Bianchi C,Becchi G,Mazzucchelli S,Corradi D,Fadini GP,Foschi D,Markmann JF,Orsi E,Škrha J,Camboni MG,Abdi R,James Shapiro AM,Folli F,Ludvigsson J,Del Prato S,Zuccotti G,Fiorina P

PA1-26938 was used in Flow Cytometry to show that the death receptor TMEM219 is expressed on pancreatic beta cells and that signaling through its ligand insulin-like growth factor binding protein 3 (IGFBP3) leads to beta cell loss and dysfunction.

Thu Feb 03 00:00:00 EST 2022

A developmental lineage-based gene co-expression network for mouse pancreatic β-cells reveals a role for Zfp800 in pancreas development.

Development (Cambridge, England)

Osipovich AB,Dudek KD,Greenfest-Allen E,Cartailler JP,Manduchi E,Potter Case L,Choi E,Chapman AG,Clayton HW,Gu G,Stoeckert CJ,Magnuson MA

PA1-26938 was used in Immunohistochemistry to demonstrate that developmentally oriented GCNs have utility for gaining new insights into gene regulation during organogenesis.

Sun Mar 21 00:00:00 EDT 2021

Insulinoma-derived pseudo-islets for diabetes research.

American journal of physiology. Cell physiology

Hart NJ,Weber C,Price N,Banuelos A,Schultz M,Huey B,Harnois E,Gibson C,Steyn LV,Papas KK,Lynch RM

PA1-26938 was used in Immunocytochemistry-immunoflourescence to conclude that INS832/3-derived IPIs provide a valuable and convenient model for accelerating islet and diabetes research.

Sun Aug 01 00:00:00 EDT 2021

Maternal adaptations of pancreatic islets and glucose metabolism after lactation.

The Journal of endocrinology

Canul-Medina G,Riverón-Negrete L,Pastén-Hidalgo K,Morales-Castillo P,García-Vázquez F,Fernandez-Mejia C

PA1-26938 was used in Immunohistochemistry to investigate the effects of post-lactation on the pancreatic islets, glucose homeostasis and concentrations of GLU-osteocalcin.

Fri Jan 01 00:00:00 EST 2021

Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes.

Molecular metabolism

Oppenländer L,Palit S,Stemmer K,Greisle T,Sterr M,Salinno C,Bastidas-Ponce A,Feuchtinger A,Böttcher A,Theis FJ,Lickert H

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunohistochemistry

Wed Dec 01 00:00:00 EST 2021

Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes.

Molecular metabolism

Oppenländer L,Palit S,Stemmer K,Greisle T,Sterr M,Salinno C,Bastidas-Ponce A,Feuchtinger A,Böttcher A,Theis FJ,Lickert H

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunohistochemistry

Wed Dec 01 00:00:00 EST 2021

Insm1, Neurod1, and Pax6 promote murine pancreatic endocrine cell development through overlapping yet distinct RNA transcription and splicing programs.

G3 (Bethesda, Md.)

Dudek KD,Osipovich AB,Cartailler JP,Gu G,Magnuson MA

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunohistochemistry

Tue Oct 19 00:00:00 EDT 2021

Scrt1, a transcriptional regulator of β-cell proliferation identified by differential chromatin accessibility during islet maturation.

Scientific reports

Sobel J,Guay C,Elhanani O,Rodriguez-Trejo A,Stoll L,Menoud V,Jacovetti C,Walker MD,Regazzi R

PA1-26938 was used in Flow Cytometry, Immunocytochemistry to identify several known and unforeseen key transcriptional events occurring during β-cell maturation.

Thu Apr 22 00:00:00 EDT 2021

Scrt1, a transcriptional regulator of β-cell proliferation identified by differential chromatin accessibility during islet maturation.

Scientific reports

Sobel J,Guay C,Elhanani O,Rodriguez-Trejo A,Stoll L,Menoud V,Jacovetti C,Walker MD,Regazzi R

PA1-26938 was used in Flow Cytometry, Immunocytochemistry to identify several known and unforeseen key transcriptional events occurring during β-cell maturation.

Thu Apr 22 00:00:00 EDT 2021

Scrt1, a transcriptional regulator of β-cell proliferation identified by differential chromatin accessibility during islet maturation.

Scientific reports

Sobel J,Guay C,Elhanani O,Rodriguez-Trejo A,Stoll L,Menoud V,Jacovetti C,Walker MD,Regazzi R

PA1-26938 was used in Flow Cytometry, Immunocytochemistry to identify several known and unforeseen key transcriptional events occurring during β-cell maturation.

Thu Apr 22 00:00:00 EDT 2021

Integrated Array Tomography for 3D Correlative Light and Electron Microscopy.

Frontiers in molecular biosciences

Lane R,Wolters AHG,Giepmans BNG,Hoogenboom JP

PA1-26938 was used in Miscellaneous to conduct a proof-of-concept of our integrated workflow on immunolabelled serial sections of tissues.

Tue Feb 08 00:00:00 EST 2022

A monoclonal antibody with broad specificity for the ligands of insulin B:9-23 reactive T cells prevents spontaneous type 1 diabetes in mice.

mAbs

Cepeda JR,Sekhar NS,Han J,Xiong W,Zhang N,Yu L,Dai S,Davidson HW,Kappler JW,An Z,Zhang L

PA1-26938 was used in Immunohistochemistry to report an enhanced reagent, mAb757, with improved specificity, affinity, and efficacy in modulating T1D.

Mon Sep 20 00:00:00 EDT 2021

Oligomeric collagen as an encapsulation material for islet/β-cell replacement: effect of islet source, dose, implant site, and administration format.

American journal of physiology. Endocrinology and metabolism

Stephens CH,Morrison RA,McLaughlin M,Orr K,Tersey SA,Scott-Moncrieff JC,Mirmira RG,Considine RV,Voytik-Harbin S

PA1-26938 was used in Immunohistochemistry to find that xenogeneic rat islets functioned similarly to or better than allogeneic mouse islets, with only modest improvements in longevity noted with dosage.

Sat Aug 01 00:00:00 EDT 2020

Macrophage IFN-I signaling promotes autoreactive T cell infiltration into islets in type 1 diabetes model.

JCI insight

Marro BS,Legrain S,Ware BC,Oldstone MB

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunofluorescence

Thu Jan 24 00:00:00 EST 2019

Macrophage IFN-I signaling promotes autoreactive T cell infiltration into islets in type 1 diabetes model.

JCI insight

Marro BS,Legrain S,Ware BC,Oldstone MB

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunofluorescence

Thu Jan 24 00:00:00 EST 2019

In situ type I oligomeric collagen macroencapsulation promotes islet longevity and function in vitro and in vivo.

American journal of physiology. Endocrinology and metabolism

Stephens CH,Orr KS,Acton AJ,Tersey SA,Mirmira RG,Considine RV,Voytik-Harbin SL

PA1-26938 was used in Immunohistochemistry-immunofluorescence to demonstrate how oligomer-islet macroencapsulation can prolong the health and function of cultured islets and has potential for a subcutaneous injectable transplantation strategy for treatment of type 1 diabetes.

Mon Oct 01 00:00:00 EDT 2018

GRP94 Is an Essential Regulator of Pancreatic β-Cell Development, Mass, and Function in Male Mice.

Endocrinology

Kim DS,Song L,Wang J,Wu H,Gu G,Sugi Y,Li Z,Wang H

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunohistochemistry

Thu Feb 01 00:00:00 EST 2018

Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice.

JCI insight

Chartoumpekis DV,Yagishita Y,Fazzari M,Palliyaguru DL,Rao UN,Zaravinos A,Khoo NK,Schopfer FJ,Weiss KR,Michalopoulos GK,Sipula I,O'Doherty RM,Kensler TW,Wakabayashi N

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunofluorescence

Thu Mar 08 00:00:00 EST 2018

Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice.

JCI insight

Chartoumpekis DV,Yagishita Y,Fazzari M,Palliyaguru DL,Rao UN,Zaravinos A,Khoo NK,Schopfer FJ,Weiss KR,Michalopoulos GK,Sipula I,O'Doherty RM,Kensler TW,Wakabayashi N

Published figure using Insulin polyclonal antibody (Product # PA1-26938) in Immunofluorescence

Thu Mar 08 00:00:00 EST 2018

Long noncoding RNAs are dynamically regulated during β-cell mass expansion in mouse pregnancy and control β-cell proliferation in vitro.

PloS one

Sisino G,Zhou AX,Dahr N,Sabirsh A,Soundarapandian MM,Perera R,Larsson-Lekholm E,Magnone MC,Althage M,Tyrberg B

PA1-26938 was used in Functional assays to test the hypothesis that IncRNAs regulate β-cell proliferation in response to prolactin in the context of β-cell mass compensation in pregnancy.

Fri Oct 06 00:00:00 EDT 2017

β-arrestin-2 is an essential regulator of pancreatic β-cell function under physiological and pathophysiological conditions.

Nature communications

Zhu L,Almaça J,Dadi PK,Hong H,Sakamoto W,Rossi M,Lee RJ,Vierra NC,Lu H,Cui Y,McMillin SM,Perry NA,Gurevich VV,Lee A,Kuo B,Leapman RD,Matschinsky FM,Doliba NM,Urs NM,Caron MG,Jacobson DA,Caicedo A,Wess J

PA126938 was used in immunohistochemistry - paraffin section to report that inactivation of the beta-arrestin-2 gene, barr2, in beta-cells greatly impairs insulin release and glucose tolerance in a calorie-rich diet

Wed Feb 01 00:00:00 EST 2017

Hypothyroidism Impairs Human Stem Cell-Derived Pancreatic Progenitor Cell Maturation in Mice.

Diabetes

Bruin JE,Saber N,O'Dwyer S,Fox JK,Mojibian M,Arora P,Rezania A,Kieffer TJ

PA1-26938 was used in immunohistochemistry - paraffin section to assess the impact of thyroid dysregulation on the development of human embryonic stem cells-derived progenitor cells in vivo

Sun May 01 00:00:00 EDT 2016

Hypothyroidism Impairs Human Stem Cell-Derived Pancreatic Progenitor Cell Maturation in Mice.

Diabetes

Bruin JE,Saber N,O'Dwyer S,Fox JK,Mojibian M,Arora P,Rezania A,Kieffer TJ

PA1-26938 was used in immunohistochemistry - paraffin section to assess the impact of thyroid dysregulation on the development of human embryonic stem cells-derived progenitor cells in vivo

Sun May 01 00:00:00 EDT 2016

Characterization of age-associated alterations of islet function and structure in diabetic mutant cryptochrome 1 transgenic mice.

Journal of diabetes investigation

Okano S,Hayasaka K,Igarashi M,Togashi Y,Nakajima O

PA1-26938 was used in immunohistochemistry to study temporal changes in pancreatic islet biology in transgenic mice expressing a mutant form of cryptochrome-1 associated with the development of diabetes

Fri Sep 13 00:00:00 EDT 2013

Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice.

The Journal of biological chemistry

Yano M,Watanabe K,Yamamoto T,Ikeda K,Senokuchi T,Lu M,Kadomatsu T,Tsukano H,Ikawa M,Okabe M,Yamaoka S,Okazaki T,Umehara H,Gotoh T,Song WJ,Node K,Taguchi R,Yamagata K,Oike Y

PA1-26938 was used in immunohistochemistry to investigate the role of sphingomyelin synthase 1 in mitochondrial function and the intracellular balance of reactive oxygen species

Fri Feb 04 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Ins; Insulin; Insulin-1; Insulin-2; preproinsulin; proinsulin

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Gene Aliases: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; proinsulin

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UniProt ID: (Human) P01308, (Mouse) P01325, (Mouse) P01326

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Entrez Gene ID: (Human) 3630, (Mouse) 16333, (Mouse) 16334

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Function(s)

protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding chaperone binding protein complex binding growth factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Insulin Polyclonal Antibody

Insulin Antibody (PA1-26938) in IHC (P)

Product Details

PA1-26938

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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