Proteintech

Insulin Monoclonal Antibody (4B6A7)

Insulin Antibody in Immunocytochemistry (ICC/IF)

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Insulin Antibody (66198-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using (Product # 66198-1-IG) (Insulin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The α cells of pancreas was labelled in green with 15954-1-AP (Glucagon antibody).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

66198-1-IG

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:10,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

Immunocytochemistry (ICC/IF)

1:100

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

4B6A7

Immunogen

Insulin Fusion Protein Ag8630 (1-110 aa encoded by BC005255)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.23 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MALWMRLLP LLALLALWGP DPAAAFVNQH LCGSHLVEAL YLVCGERGFF YTPKTRREAE DLQVGQVELG GGPGAGSLQP LALEGSLQKR GIVEQCCTSI CSLYQLENYC N (1-110 aa encoded by BC005255)

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fufang-zhenzhu-tiaozhi formula protects islet against injury and promotes β cell regeneration in diabetic mice.

Journal of ethnopharmacology

Chen X,Yin J,Zhong Q,Wang K,Zhang X,Liang M,Lin Q,Wang H,Wang W,Wang L,Hu X,Bei W,Guo J

Mon Jan 30 00:00:00 EST 2023

Effects of gradient high-field static magnetic fields on diabetic mice.

Zoological research

Yu B,Song C,Feng CL,Zhang J,Wang Y,Zhu YM,Zhang L,Ji XM,Tian XF,Cheng GF,Chen WL,Zablotskii V,Wang H,Zhang X

Sat Mar 18 00:00:00 EDT 2023

NAG-1/GDF15 protects against streptozotocin-induced type 1 diabetes by inhibiting apoptosis, preserving beta-cell function, and suppressing inflammation in pancreatic islets.

Molecular and cellular endocrinology

Wang Y,Chen J,Sang T,Chen C,Peng H,Lin X,Zhao Q,Chen S,Eling T,Wang X

Wed Jun 01 00:00:00 EDT 2022

SDF-1 inhibits the dedifferentiation of islet β cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4.

Journal of cellular and molecular medicine

Chen XY,Shi YX,Huang YP,Ding M,Shen QL,Li CJ,Lin JN

Tue Feb 01 00:00:00 EST 2022

Type IIB PKA is highly expressed in β cells and controls cell proliferation via regulating Cyclin D1 expression.

The FEBS journal

Jiang Y,Zhu L,Wu D,Ni Y,Huang C,Ye H,Yang Y,Liu R,Li Y

Sun May 01 00:00:00 EDT 2022

Nicotinamide mononucleotide promotes pancreatic islet function through the SIRT1 pathway in mice after severe burns.

Burns : journal of the International Society for Burn Injuries

Liu X,Li D,Liu Z,Song Y,Zhang B,Zang Y,Zhang W,Niu Y,Shen C

Thu Dec 01 00:00:00 EST 2022

Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.

Food chemistry: X

Fang J,Lin Y,Xie H,Farag MA,Feng S,Li J,Shao P

Wed Mar 30 00:00:00 EDT 2022

cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hu H,Zhao R,He Q,Cui C,Song J,Guo X,Zang N,Yang M,Zou Y,Yang J,Li J,Wang L,Xia L,Wang L,He F,Hou X,Yan F,Chen L

Sun May 01 00:00:00 EDT 2022

Metabolic effects of CCL5 deficiency in lean and obese mice.

Frontiers in immunology

Zhou H,Liao X,Zeng Q,Zhang H,Song J,Hu W,Sun X,Ding Y,Wang D,Xiao Y,Deng T

Wed Feb 01 00:00:00 EST 2023

Troxerutin attenuates insulin resistance via pancreatic IL-22/JAK1/STAT3 signaling activation in dihydrotestosterone-induced polycystic ovary syndrome rats.

American journal of physiology. Endocrinology and metabolism

Gao Z,Wang G,Ma X,Tan H,Zhang C,Yin X,Suo F,Yao R,Yan X

Tue Nov 01 00:00:00 EDT 2022

Short-Clustered Maltodextrin Activates Ileal Glucose-Sensing and Induces Glucagon-like Peptide 1 Secretion to Ameliorate Glucose Homeostasis in Type 2 Diabetic Mice.

Journal of agricultural and food chemistry

Kong H,Yu L,Li C,Ban X,Gu Z,Li Z

Wed Oct 05 00:00:00 EDT 2022

Proinflammatory signaling in islet β cells propagates invasion of pathogenic immune cells in autoimmune diabetes.

Cell reports

Piñeros AR,Kulkarni A,Gao H,Orr KS,Glenn L,Huang F,Liu Y,Gannon M,Syed F,Wu W,Anderson CM,Evans-Molina C,McDuffie M,Nadler JL,Morris MA,Mirmira RG,Tersey SA

Tue Jun 28 00:00:00 EDT 2022

Green light exposure aggravates high-fat diet feeding-induced hepatic steatosis and pancreatic dysfunction in male mice.

Ecotoxicology and environmental safety

Zhang S,Xu M,Shen Z,Shang C,Zhang W,Chen S,Liu C

Wed Dec 01 00:00:00 EST 2021

Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis.

Cell reports

Zhang X,Wang X,Yuan Z,Radford SJ,Liu C,Libutti SK,Zheng XFS

Tue Mar 16 00:00:00 EDT 2021

Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.

Molecular and cellular endocrinology

Guo X,Cui C,Song J,He Q,Zang N,Hu H,Wang X,Li D,Wang C,Hou X,Li X,Liang K,Yan F,Chen L

Mon Nov 01 00:00:00 EDT 2021

Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model.

The Journal of biological chemistry

Fujimoto BA,Young M,Nakamura N,Ha H,Carter L,Pitts MW,Torres D,Noh HL,Suk S,Kim JK,Polgar N

Fri Aug 27 00:00:00 EDT 2021

Lipoxin A4 promotes adipogenic differentiation and browning of mouse embryonic fibroblasts.

In vitro cellular & developmental biology. Animal

Wang Q,Jin F,Zhang J,Li Z,Yu D

Wed Dec 01 00:00:00 EST 2021

Stomach secretes estrogen in response to the blood triglyceride levels.

Communications biology

Ito T,Yamamoto Y,Yamagishi N,Kanai Y

Tue Dec 07 00:00:00 EST 2021

Mesenchymal stem cell-conditioned medium alleviates high fat-induced hyperglucagonemia via miR-181a-5p and its target PTEN/AKT signaling.

Molecular and cellular endocrinology

Song J,He Q,Guo X,Wang L,Wang J,Cui C,Hu H,Yang M,Cui Y,Zang N,Yan F,Liu F,Sun Y,Liang K,Qin J,Zhao R,Wang C,Sun Z,Hou X,Li W,Chen L

Mon Nov 01 00:00:00 EDT 2021

Novel Short-Clustered Maltodextrin as a Dietary Starch Substitute Attenuates Metabolic Dysregulation and Restructures Gut Microbiota in db/db Mice.

Journal of agricultural and food chemistry

Kong H,Yu L,Gu Z,Li C,Ban X,Cheng L,Hong Y,Li Z

Wed Nov 04 00:00:00 EST 2020

Novel Short-Clustered Maltodextrin as a Dietary Starch Substitute Attenuates Metabolic Dysregulation and Restructures Gut Microbiota in db/db Mice.

Journal of agricultural and food chemistry

Kong H,Yu L,Gu Z,Li C,Ban X,Cheng L,Hong Y,Li Z

Wed Nov 04 00:00:00 EST 2020

Mitomycin C treatment improves pancreatic islet graft longevity in intraportal islet transplantation by suppressing proinflammatory response.

Scientific reports

Yamane K,Anazawa T,Tada S,Fujimoto N,Inoguchi K,Emoto N,Nagai K,Masui T,Okajima H,Takaori K,Sumi S,Uemoto S

Tue Jul 21 00:00:00 EDT 2020

Involvement of Estrogen Receptor-α in the Activation of Nrf2-Antioxidative Signaling Pathways by Silibinin in Pancreatic β-Cells.

Biomolecules & therapeutics

Chu C,Gao X,Li X,Zhang X,Ma R,Jia Y,Li D,Wang D,Xu F

Sun Mar 01 00:00:00 EST 2020

Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.

Cell death & disease

Zhang J,An H,Ni K,Chen B,Li H,Li Y,Sheng G,Zhou C,Xie M,Chen S,Zhou T,Yang G,Chen X,Wu G,Jin S,Li M

Thu Apr 11 00:00:00 EDT 2019

Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yang G,Yan R,Tong H,Zhang J,Chen B,Xue X,Wang J,Chu M,Jin S,Li M

Fri Mar 18 00:00:00 EDT 2022

Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yang G,Yan R,Tong H,Zhang J,Chen B,Xue X,Wang J,Chu M,Jin S,Li M

Fri Mar 18 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: Ins; Insulin; Insulin-1; Insulin-2; preproinsulin; proinsulin

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Gene Aliases: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; proinsulin

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UniProt ID: (Human) P01308, (Mouse) P01325, (Mouse) P01326

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Entrez Gene ID: (Human) 3630, (Mouse) 16333, (Mouse) 16334

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Function(s)

protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding chaperone binding protein complex binding growth factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Insulin Monoclonal Antibody (4B6A7)

Insulin Antibody (66198-1-IG) in ICC/IF

Product Details

66198-1-IG

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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