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Invitrogen
Insulin Monoclonal Antibody (ICBTACLS), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 3
Insulin Antibody (53-9769-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human pancreas using 5 µg/mL Anti-Insulin Alexa Fluor® 488 (green), 5 µg/mL Anti-Somatostatin eFluor® 660 (white), and 2.5 µg/mL Anti-Glucagon Biotin followed by 1 µg/mL Streptavidin eFluor® 570 (red). Nuclei are stained with DAPI (blue).
Product Details
53-9769-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ICBTACLS
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574469
Product Specific Information
Description: This ICBTACLS monoclonal antibody reacts with the hormone insulin, responsible for regulation of carbohydrate and fat metabolism. Blood glucose levels are maintained by insulin. Insulin secretion from pancreatic beta-cells within the islets of Langerhans results in uptake of excess glucose from the blood into organs and its storage in the form of glycogen. Patients with type 1 diabetes have an autoimmune destruction of insulin-producing beta-cells in the pancreas, resulting in insulin deficiency and elevated levels of blood glucose. Patients with type 2 diabetes have insulin resistance where insulin becomes less effective at reducing the amount of blood glucose. Insulin is also important for proper endothelial cell function in with respect to vascular elasticity in response to changing blood pressure and plays a role in cognition, learning, and memory.
The ICBTACLS antibody recognizes human, mouse, and rat insulin.
Applications Reported: This ICBTACLS antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This ICBTACLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ins; Insulin; Insulin-1; Insulin-2; preproinsulin; proinsulin
Gene Aliases: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; proinsulin
UniProt ID: (Human) P01308, (Rat) P01322, (Mouse) P01325, (Rat) P01323, (Mouse) P01326
Entrez Gene ID: (Human) 3630, (Rat) 24505, (Mouse) 16333, (Rat) 24506, (Mouse) 16334
MAPK cascade
negative regulation of acute inflammatory response
glucose metabolic process
regulation of transcription, DNA-templated
regulation of cellular amino acid metabolic process
ER to Golgi vesicle-mediated transport
acute-phase response
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of gene expression
positive regulation of phosphatidylinositol 3-kinase signaling
glucose transport
regulation of transmembrane transporter activity
positive regulation of cell growth
positive regulation of cell migration
positive regulation of protein autophosphorylation
activation of protein kinase B activity
positive regulation of cellular protein metabolic process
negative regulation of protein oligomerization
regulation of protein localization
negative regulation of NAD(P)H oxidase activity
wound healing
negative regulation of protein catabolic process
glucose homeostasis
positive regulation of MAPK cascade
cellular protein metabolic process
positive regulation of nitric oxide biosynthetic process
positive regulation of cell differentiation
negative regulation of gluconeogenesis
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
negative regulation of glycogen catabolic process
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
negative regulation of proteolysis
negative regulation of vasodilation
positive regulation of vasodilation
negative regulation of fatty acid metabolic process
positive regulation of glucose import
positive regulation of insulin receptor signaling pathway
alpha-beta T cell activation
positive regulation of lipid biosynthetic process
regulation of protein secretion
negative regulation of protein secretion
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of lipid catabolic process
positive regulation of nitric-oxide synthase activity
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
fatty acid homeostasis
negative regulation of respiratory burst involved in inflammatory response
positive regulation of respiratory burst
positive regulation of peptide hormone secretion
positive regulation of brown fat cell differentiation
positive regulation of protein localization to nucleus
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of feeding behavior
response to organic substance
response to cytokine
response to peptide hormone
response to cAMP
carbohydrate metabolic process
negative regulation of transcription from RNA polymerase II promoter
activation of MAPK activity
ER overload response
myoblast fusion
biological_process
lipid catabolic process
regulation of protein binding
positive regulation of phosphatidylinositol 3-kinase activity
canonical Wnt signaling pathway
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
activation of JUN kinase activity
regulation of gene expression
myotube differentiation
regulation of phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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