Proteintech

JNK Polyclonal Antibody

JNK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

JNK Antibody (10023-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10023-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

JNK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

JNK Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10023-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 26 publications 26 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

JNK Fusion Protein Ag0019

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

MAPK8 can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot.

Target Information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Young Sca-1⁺ bone marrow stem cell-derived exosomes preserve visual function via the miR-150-5p/MEKK3/JNK/c-Jun pathway to reduce M1 microglial polarization.

Journal of nanobiotechnology

Wang Y,Qin WY,Wang Q,Liu XN,Li XH,Ye XQ,Bai Y,Zhang Y,Liu P,Wang XL,Zhou YH,Yuan HP,Shao ZB

Thu Jun 15 00:00:00 EDT 2023

EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus.

Antioxidants (Basel, Switzerland)

Kim JE,Kim TH,Kang TC

Thu Apr 20 00:00:00 EDT 2023

TYRO3 protects podocyte via JNK/c-jun-P53 pathway.

Archives of biochemistry and biophysics

Zhang L,Jiang S,Shi J,Xu X,Wang L,Zhai X,Hou Q,Qin W,Chen Z

Mon May 01 00:00:00 EDT 2023

Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation.

The Journal of biological chemistry

Byts N,Sharma S,Malm T,Kaakinen M,Korhonen P,Jaakkonen L,Keuters M,Huuskonen M,Pietilä I,Koistinaho J,Koivunen P,Myllyharju J

Tue Mar 01 00:00:00 EST 2022

Astragalin mitigates inflammatory osteolysis by negatively modulating osteoclastogenesis via ROS and MAPK signaling pathway.

International immunopharmacology

Xing F,Geng L,Guan H,Liu D,Li Y,Zeng L,Chen Y,Tian R,Li Z,Cao R,Zhao Y,Yan P,Qiang H,Kong N,Wang K,Yang P

Tue Nov 01 00:00:00 EDT 2022

Cangfudaotan decoction inhibits mitochondria-dependent apoptosis of granulosa cells in rats with polycystic ovarian syndrome.

Frontiers in endocrinology

Jiang XL,Tai H,Xiao XS,Zhang SY,Cui SC,Qi SB,Hu DD,Zhang LN,Kuang JS,Meng XS,Li SM

Tue Dec 06 00:00:00 EST 2022

BCATc inhibitor 2 ameliorated mitochondrial dysfunction and apoptosis in oleic acid-induced non-alcoholic fatty liver disease model.

Frontiers in pharmacology

Lu Z,Sun GF,Pan XA,Qu XH,Yang P,Chen ZP,Han XJ,Wang T

Fri Nov 18 00:00:00 EST 2022

Excretory/secretory proteins inhibit host immune responses by downregulating the TLR4/NF-κB/MAPKs signaling pathway: A possible mechanism of immune evasion in parasitic nematode Haemonchus contortus.

Frontiers in immunology

Wen Z,Zhang Y,Feng J,Aimulajiang K,Aleem MT,Lu M,Xu L,Song X,Li X,Yan R

Mon Oct 17 00:00:00 EDT 2022

Isovitexin Inhibits Ginkgolic Acids-Induced Inflammation Through Downregulating SHP2 Activation.

Frontiers in pharmacology

Zhang Y,Qi Z,Wang W,Wang L,Cao F,Zhao L,Fang X

Tue Aug 31 00:00:00 EDT 2021

The anti-rotavirus effect of baicalin via the gluconeogenesis-related p-JNK-PDK1-AKT-SIK2 signaling pathway.

European journal of pharmacology

Song L,Zhong P,Zhu X,Zhou R,Gao M,Lan Q,Chen J,Chen Y,Zhao W

Thu Apr 15 00:00:00 EDT 2021

A new prognosis prediction model combining TNM stage with MAP2K4 and JNK in postoperative pancreatic cancer patients.

Pathology, research and practice

Liu XD,Zhang ZW,Wu HW,Liang ZY

Fri Jan 01 00:00:00 EST 2021

A new prognosis prediction model combining TNM stage with MAP2K4 and JNK in postoperative pancreatic cancer patients.

Pathology, research and practice

Liu XD,Zhang ZW,Wu HW,Liang ZY

Fri Jan 01 00:00:00 EST 2021

PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats.

Ecotoxicology and environmental safety

Yang Y,Feng Y,Huang H,Cui L,Li F

Mon Mar 15 00:00:00 EDT 2021

Overexpression of FOXA2 attenuates cigarette smoke-induced cellular senescence and lung inflammation through inhibition of the p38 and Erk1/2 MAPK pathways.

International immunopharmacology

Tao Y,Sun Y,Wu B,Xu D,Yang J,Gu L,Du C

Sat May 01 00:00:00 EDT 2021

Synergistic effect of Huyang Yangkun Formula and embryonic stem cells on 4-vinylcyclohexene diepoxide induced premature ovarian insufficiency in mice.

Chinese medicine

Li M,Xie L,Li Y,Liu J,Nie G,Yang H

Sat Apr 16 00:00:00 EDT 2022

Synergistic effect of Huyang Yangkun Formula and embryonic stem cells on 4-vinylcyclohexene diepoxide induced premature ovarian insufficiency in mice.

Chinese medicine

Li M,Xie L,Li Y,Liu J,Nie G,Yang H

Sat Apr 16 00:00:00 EDT 2022

Phenolic Glycosides from the Roots of Ficus hirta Vahl. and Their Antineuroinflammatory Activities.

Journal of agricultural and food chemistry

Ye X,Tian W,Wang G,Zhang X,Zhou M,Zeng D,Liu X,Yao X,Zhang Y,Chen H

Wed Apr 08 00:00:00 EDT 2020

Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis.

Pharmacological research

Wei Y,Meng M,Tian Z,Xie F,Yin Q,Dai C,Wang J,Zhang Q,Liu Y,Liu C,Yan F,Jiang F,Guo X

Fri Mar 01 00:00:00 EST 2019

Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis.

Pharmacological research

Wei Y,Meng M,Tian Z,Xie F,Yin Q,Dai C,Wang J,Zhang Q,Liu Y,Liu C,Yan F,Jiang F,Guo X

Fri Mar 01 00:00:00 EST 2019

Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.

Frontiers in oncology

Lin DD,Shen Y,Qiao S,Liu WW,Zheng L,Wang YN,Cui N,Wang YF,Zhao S,Shi JH

Thu Oct 01 00:00:00 EDT 2020

The Regional Specific Alterations in BBB Permeability are Relevant to the Differential Responses of 67-kDa LR Expression in Endothelial Cells and Astrocytes Following Status Epilepticus.

International journal of molecular sciences

Park H,Kang TC

Fri Nov 29 00:00:00 EST 2019

CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.

Cells

Kim JE,Park H,Choi SH,Kong MJ,Kang TC

Mon Aug 05 00:00:00 EDT 2019

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Theriogenology

Wang X,Adegoke EO,Ma M,Huang F,Zhang H,Adeniran SO,Zheng P,Zhang G

Tue Oct 15 00:00:00 EDT 2019

Blockade of AMPA Receptor Regulates Mitochondrial Dynamics by Modulating ERK1/2 and PP1/PP2A-Mediated DRP1-S616 Phosphorylations in the Normal Rat Hippocampus.

Frontiers in cellular neuroscience

Kim JE,Choi HC,Song HK,Kang TC

Thu Oct 01 00:00:00 EDT 2020

Melatonin reprogramming of gut microbiota improves lipid dysmetabolism in high-fat diet-fed mice.

Journal of pineal research

Yin J,Li Y,Han H,Chen S,Gao J,Liu G,Wu X,Deng J,Yu Q,Huang X,Fang R,Li T,Reiter RJ,Zhang D,Zhu C,Zhu G,Ren W,Yin Y

Thu Nov 01 00:00:00 EDT 2018

Melatonin reprogramming of gut microbiota improves lipid dysmetabolism in high-fat diet-fed mice.

Journal of pineal research

Yin J,Li Y,Han H,Chen S,Gao J,Liu G,Wu X,Deng J,Yu Q,Huang X,Fang R,Li T,Reiter RJ,Zhang D,Zhu C,Zhu G,Ren W,Yin Y

Thu Nov 01 00:00:00 EDT 2018

All-Trans Retinoic Acid Ameliorates the Early Experimental Cerebral Ischemia-Reperfusion Injury in Rats by Inhibiting the Loss of the Blood-Brain Barrier via the JNK/P38MAPK Signaling Pathway.

Neurochemical research

Li M,Tian X,An R,Yang M,Zhang Q,Xiang F,Liu H,Wang Y,Xu L,Dong Z

Fri Jun 01 00:00:00 EDT 2018

All-Trans Retinoic Acid Ameliorates the Early Experimental Cerebral Ischemia-Reperfusion Injury in Rats by Inhibiting the Loss of the Blood-Brain Barrier via the JNK/P38MAPK Signaling Pathway.

Neurochemical research

Li M,Tian X,An R,Yang M,Zhang Q,Xiang F,Liu H,Wang Y,Xu L,Dong Z

Fri Jun 01 00:00:00 EDT 2018

TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation.

Neuropharmacology

Ko AR,Kang TC

Sat Jul 15 00:00:00 EDT 2017

TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation.

Neuropharmacology

Ko AR,Kang TC

Sat Jul 15 00:00:00 EDT 2017

Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release.

Chemical research in toxicology

Xu D,Su C,Song X,Shi Q,Fu J,Hu L,Xia X,Song E,Song Y

Mon Jun 15 00:00:00 EDT 2015

Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release.

Chemical research in toxicology

Xu D,Su C,Song X,Shi Q,Fu J,Hu L,Xia X,Song E,Song Y

Mon Jun 15 00:00:00 EDT 2015

13-acetoxysarcocrassolide induces apoptosis on human gastric carcinoma cells through mitochondria-related apoptotic pathways: p38/JNK activation and PI3K/AKT suppression.

Marine drugs

Su CC,Chen JY,Din ZH,Su JH,Yang ZY,Chen YJ,Wang RY,Wu YJ

Thu Oct 23 00:00:00 EDT 2014

13-acetoxysarcocrassolide induces apoptosis on human gastric carcinoma cells through mitochondria-related apoptotic pathways: p38/JNK activation and PI3K/AKT suppression.

Marine drugs

Su CC,Chen JY,Din ZH,Su JH,Yang ZY,Chen YJ,Wang RY,Wu YJ

Thu Oct 23 00:00:00 EDT 2014

Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis.

Chinese medical journal

Lin CL,Zhang ZX,Xu YJ,Ni W,Chen SX

Wed Jan 05 00:00:00 EST 2005

Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis.

Chinese medical journal

Lin CL,Zhang ZX,Xu YJ,Ni W,Chen SX

Wed Jan 05 00:00:00 EST 2005

Bioinformatics

Protein Aliases: c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK 46; JNK 55; JNK-46; JNK-55; JNK/SAPK alpha; JNK1 beta1 protein kinase; JNK3 alpha protein kinase; JNK3 beta1 protein kinase; JNK3 beta2 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 10; MAPK 8; MAPK 9; MGC50974; Mi; mitogen activated protein kinase 10; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 10; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; Mitogen-activated protein kinase 9; MK10; p54 gamma; p54-alpha; p54-beta; Phospho-JNK; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK gamma; SAPK-alpha; SAPK-beta; SAPK/Erk/kinase 2; SAPK1a; SAPK1b; SAPK1c; stress activated protein kinase alpha II; Stress activated protein kinase beta; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1b; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2; Stress-activated protein kinase JNK3

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Gene Aliases: AI849689; AI851083; C230008H04Rik; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; JNK3B1; JNK3B2; MAPK10; MAPK8; MAPK9; p493F12; p54a; p54aSAPK; p54bSAPK; PRKM10; PRKM8; PRKM9; SAPb; SAPK; SAPK(beta); SAPK1; SAPK1A; SAPK1B; SAPK1C; SAPKC; Serk2

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UniProt ID: (Human) P53779, (Human) P45983, (Human) P45984, (Mouse) Q61831, (Rat) P49187, (Rat) P49185, (Mouse) Q91Y86, (Rat) P49186, (Mouse) Q9WTU6

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Entrez Gene ID: (Human) 5602, (Human) 5599, (Human) 5601, (Mouse) 26414, (Rat) 25272, (Rat) 116554, (Mouse) 26419, (Rat) 50658, (Mouse) 26420

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Function(s)

JUN kinase activity MAP kinase kinase activity protein binding ATP binding protein serine/threonine kinase activity kinase activity enzyme binding histone deacetylase regulator activity histone deacetylase binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process mitogen-activated protein kinase kinase kinase binding nucleotide binding protein kinase activity MAP kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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JNK Polyclonal Antibody

JNK Antibody (10023-1-AP) in ICC/IF

Product Details

10023-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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