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Product Details
MA3-933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Guinea pig,
Mouse,
Rabbit,
Rat
Published species
Pig,
Rabbit
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
VF1c
Immunogen
Rabbit skeletal muscle triads.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_325516
Product Specific Information
MA3-933 detects the junctional sarcoplasmic reticulum (SR) protein from rabbit and guinea pig; moderately in canine and rat; and weakly in mouse.
MA3-933 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~90 kDa protein representing junctional SR in rabbit skeletal muscle extracts. Immunofluorescence staining of junctional sarcoplasmic reticulum with MA3-933 results in an increase in intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle, and does not cross-react with cardiac muscle.
The MA3-933 antigen is rabbit skeletal muscle triads.
Target Information
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR and RyR. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: junctional sarcoplasmic reticulum protein 1; Junctional SR; junctional-face membrane protein of 45 kDa homolog
Gene Aliases: 2300003C06Rik; 2310032K21Rik; JP-45; JP45
Entrez Gene ID: (Dog) 612184, (Rat) 690423, (Mouse) 71912
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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