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KARS Antibody (MA5-53042) in WB
Western Blot analysis of KARS. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KARS recombinant monoclonal antibody (Product # MA5-53042) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
Product Details
MA5-53042
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB6410
Immunogen
A synthesized peptide derived from human LysRS (550-597AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 6.1.1.6; lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl-tRNA synthetase
Gene Aliases: AA589550; AL024334; AL033315; AL033367; CMTRIB; D8Ertd698e; D8Wsu108e; DFNB89; KARS; KARS1; KARS2; KIAA0070; KRS; LysRS; mKIAA0070
UniProt ID: (Human) Q15046, (Rat) Q5XIM7, (Mouse) Q99MN1
Entrez Gene ID: (Human) 3735, (Rat) 292028, (Mouse) 85305
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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