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KIAA0391 Antibody (PA5-100619) in ICC/IF
Immunocytochemistry analysis of KIAA0391 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with KIAA0391 polyclonal antibody (Product # PA5-100619) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100619
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KIAA0391(Accession O15091), corresponding to amino acid residues I292-V342.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850126
Product Specific Information
Antibody detects endogenous levels of total MRPP3.
Target Information
KIAA0391, also known as MRPP3 (mitochondrial ribonuclease P protein 3), is a 583 amino acid protein that is located in the mitochondria. KIAA0391 functions in tRNA maturation and is a component of mitochondrial ribonuclease P which cleaves tRNA at the 5'-end. Mitochondrial ribonuclease P is an enzyme composed of RG9MTD1, MRPP22 and KIAA0391 subunits. Due to alternative splicing events, KIAA0391 exists as four isoforms. The gene encoding KIAA0391 maps to human chromosome 14, which contains about 700 genes and makes up about 3. 5% of human cellular DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mitochondrial ribonuclease P catalytic subunit; Mitochondrial ribonuclease P protein 3; Mitochondrial RNase P protein 3; mitochondrial RNase P subunit 3; Protein only RNase P catalytic subunit; proteinaceous RNase P
Gene Aliases: KIAA0391; mKIAA0391; MRPP3; PRORP
UniProt ID: (Mouse) Q8JZY4, (Human) O15091, (Rat) B5DF07
Entrez Gene ID: (Mouse) 66132, (Human) 9692, (Rat) 299050
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