Proteintech

KU70 Polyclonal Antibody

KU70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

KU70 Antibody (10723-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

KU70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KU70 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10723-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KU70 Fusion Protein Ag1043 (307-609 aa encoded by BC008343)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TGGLLLPSD TKRSQIYGSR QIILEKEETE ELKRFDDPGL MLMGFKPLVL LKKHHYLRPS LFVYPEESLV IGSSTLFSAL LIKCLEKEVA ALCRYTPRRN IPPYFVALVP QEEELDDQKI QVTPPGFQLV FLPFADDKRK MPFTEKIMAT PEQVGKMKAI VEKLRFTYRS DSFENPVLQQ HFRNLEALAL DLMEPEQAVD LTLPKVEAMN KRLGSLVDEF KELVYPPDYN PEGKVTKRKH DNEGSGSKRP KVEYSEEELK THISKGTLGK FTVPMLKEAC RAYGLKSGLK KQELLEALTK HFQD (307-609 aa encoded by B C008343)

Target Information

XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer.

Journal of ovarian research

Dey G,Bharti R,Braley C,Alluri R,Esakov E,Crean-Tate K,McCrae K,Joehlin-Price A,Rose PG,Lathia J,Gong Z,Reizes O

Tue Jun 27 00:00:00 EDT 2023

C9orf72 functions in the nucleus to regulate DNA damage repair.

Cell death and differentiation

He L,Liang J,Chen C,Chen J,Shen Y,Sun S,Li L

Wed Mar 01 00:00:00 EST 2023

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Chen M,Zhao E,Li M,Xu M,Hao S,Gao Y,Wu X,Li X,Yu Y,Yu Z,Yin Y

Tue Jul 25 00:00:00 EDT 2023

Screening and characterization of myositis-related autoantibodies in COVID-19 patients.

Clinical and translational science

Teo KF,Chen DY,Hsu JT,Lai YH,Chang CK,Hsueh PR,Lan JL,Hsu JL

Sun Jan 01 00:00:00 EST 2023

Screening and characterization of myositis-related autoantibodies in COVID-19 patients.

Clinical and translational science

Teo KF,Chen DY,Hsu JT,Lai YH,Chang CK,Hsueh PR,Lan JL,Hsu JL

Sun Jan 01 00:00:00 EST 2023

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy.

The FEBS journal

Wei Y,Xiao G,Xu H,Sun X,Shi Y,Wang F,Kang J,Peng J,Zhou F

Sat Jul 01 00:00:00 EDT 2023

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway.

Frontiers in oncology

Wen X,Chen S,Chen X,Qiu H,Wang W,Zhang N,Liu W,Wang T,Ding X,Zhang L

Wed Oct 19 00:00:00 EDT 2022

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Cell reports

Li Q,Yao H,Wang Y,Wu Y,Thorne RF,Zhu Y,Wu M,Liu L

Tue Nov 22 00:00:00 EST 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 EST 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 EST 2022

Proteomic Analysis of Vero Cells Infected with Pseudorabies Virus.

Viruses

Yang X,Xu S,Chen D,Jiang R,Kang H,Ge X,Zhou L,Han J,Zhang Y,Guo X,Yang H

Mon Apr 04 00:00:00 EDT 2022

NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.

iScience

Shen W,Luo P,Sun Y,Zhang W,Zhou N,Zhan H,Zhang Q,Shen J,Lin A,Cheng Q,Wang Q,Zhang J,Wang HH,Wei T

Fri Jun 17 00:00:00 EDT 2022

Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca²⁺]_m-PDP1-PDH-histone acetylation retrograde signaling.

Cell death & disease

Shi Y,Wang Y,Jiang H,Sun X,Xu H,Wei X,Wei Y,Xiao G,Song Z,Zhou F

Mon Sep 06 00:00:00 EDT 2021

LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.

Cell death & disease

Guo Z,Wang YH,Xu H,Yuan CS,Zhou HH,Huang WH,Wang H,Zhang W

Mon Jan 04 00:00:00 EST 2021

Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability.

The Journal of biological chemistry

Cao C,Han P,Liu L,Tang Y,Tian S,Zhang K,Shi L,Liu Z,Zhuo D,Ge W,Gong W

Wed Sep 01 00:00:00 EDT 2021

OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.

Cell death & disease

Cui Y,Xie R,Zhang X,Liu Y,Hu Y,Li Y,Liu X,Yu X,Wu C

Wed Jun 16 00:00:00 EDT 2021

Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer.

Journal of materials chemistry. B

Li S,Sun W,Luo Y,Gao Y,Jiang X,Yuan C,Han L,Cao K,Gong Y,Xie C

Wed Jun 16 00:00:00 EDT 2021

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

The international journal of biochemistry & cell biology

Li G,Zhang X,Wang H,Liu D,Li Z,Wu Z,Yang H

Sat Aug 01 00:00:00 EDT 2020

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Aiken CE,Tarry-Adkins JL,Spiroski AM,Nuzzo AM,Ashmore TJ,Rolfo A,Sutherland MJ,Camm EJ,Giussani DA,Ozanne SE

Sat Jun 01 00:00:00 EDT 2019

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

The Journal of physiology

Aiken CE,Tarry-Adkins JL,Spiroski AM,Nuzzo AM,Ashmore TJ,Rolfo A,Sutherland MJ,Camm EJ,Giussani DA,Ozanne SE

Wed May 01 00:00:00 EDT 2019

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Oncogene

Hu L,Li X,Liu Q,Xu J,Ge H,Wang Z,Wang H,Wang Z,Shi C,Xu X,Huang J,Lin Z,Pieper RO,Weng C

Thu Feb 23 00:00:00 EST 2017

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Oncogene

Hu L,Li X,Liu Q,Xu J,Ge H,Wang Z,Wang H,Wang Z,Shi C,Xu X,Huang J,Lin Z,Pieper RO,Weng C

Thu Feb 23 00:00:00 EST 2017

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Oncogene

Hu L,Li X,Liu Q,Xu J,Ge H,Wang Z,Wang H,Wang Z,Shi C,Xu X,Huang J,Lin Z,Pieper RO,Weng C

Thu Feb 23 00:00:00 EST 2017

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Oncogene

Hu L,Li X,Liu Q,Xu J,Ge H,Wang Z,Wang H,Wang Z,Shi C,Xu X,Huang J,Lin Z,Pieper RO,Weng C

Thu Feb 23 00:00:00 EST 2017

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Journal of Cancer

Wang Y,Hu L,Zhang X,Zhao H,Xu H,Wei Y,Jiang H,Xie C,Zhou Y,Zhou F

Thu Oct 01 00:00:00 EDT 2020

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Journal of Cancer

Wang Y,Hu L,Zhang X,Zhao H,Xu H,Wei Y,Jiang H,Xie C,Zhou Y,Zhou F

Thu Oct 01 00:00:00 EDT 2020

USP22 Induces Cisplatin Resistance in Lung Adenocarcinoma by Regulating γH2AX-Mediated DNA Damage Repair and Ku70/Bax-Mediated Apoptosis.

Frontiers in pharmacology

Wang A,Ning Z,Lu C,Gao W,Liang J,Yan Q,Tan G,Liu J

Tue Sep 29 00:00:00 EDT 2020

USP22 Induces Cisplatin Resistance in Lung Adenocarcinoma by Regulating γH2AX-Mediated DNA Damage Repair and Ku70/Bax-Mediated Apoptosis.

Frontiers in pharmacology

Wang A,Ning Z,Lu C,Gao W,Liang J,Yan Q,Tan G,Liu J

Tue Sep 29 00:00:00 EDT 2020

High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the β-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis.

International journal of molecular sciences

Zhu B,Cheng D,Li S,Zhou S,Yang Q

Fri Jul 22 00:00:00 EDT 2016

High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the β-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis.

International journal of molecular sciences

Zhu B,Cheng D,Li S,Zhou S,Yang Q

Fri Jul 22 00:00:00 EDT 2016

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

RNAi silencing targeting RNF8 enhances radiosensitivity of a non-small cell lung cancer cell line A549.

International journal of radiation biology

Zhou H,Mu X,Chen J,Liu H,Shi W,Xing E,Yang K,Wu G

Sun Sep 01 00:00:00 EDT 2013

RNAi silencing targeting RNF8 enhances radiosensitivity of a non-small cell lung cancer cell line A549.

International journal of radiation biology

Zhou H,Mu X,Chen J,Liu H,Shi W,Xing E,Yang K,Wu G

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CT; CTA-216E10.7; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen protein p70 homolog; Ku autoantigen, 70kDa; Ku p70; Ku70; Ku70 DNA-binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa); Lupus Ku autoantigen protein p70; OTTHUMP00000028581; thyroid autoantigen; thyroid autoantigen 70 kDa; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6

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Gene Aliases: 70kDa; CTC75; CTCBF; G22P1; KU70; Kup70; ML8; TLAA; XRCC6

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UniProt ID: (Human) P12956, (Mouse) P23475

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Entrez Gene ID: (Human) 2547, (Rat) 25019, (Mouse) 14375

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Function(s)

DNA binding damaged DNA binding double-stranded DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity protein binding ATP binding protein C-terminus binding transcription regulatory region DNA binding poly(A) RNA binding macromolecular complex binding 5'-deoxyribose-5-phosphate lyase activity telomeric DNA binding nucleotide binding catalytic activity helicase activity hydrolase activity lyase activity DNA helicase

Process(es)

telomere maintenance DNA ligation double-strand break repair via nonhomologous end joining DNA recombination transcription, DNA-templated positive regulation of type I interferon production DNA duplex unwinding negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of smooth muscle cell proliferation protein heterotetramerization cellular response to gamma radiation cellular response to X-ray establishment of integrated proviral latency double-strand break repair via classical nonhomologous end joining double-strand break repair cellular response to DNA damage stimulus brain development response to ionizing radiation V(D)J recombination positive regulation of neurogenesis cellular hyperosmotic salinity response DNA repair regulation of transcription, DNA-templated metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KU70 Polyclonal Antibody

KU70 Antibody (10723-1-AP) in ICC/IF

Product Details

10723-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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