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Ku70 Antibody (MA5-35083) in ICC/IF
Confocal imaging (Immunocytochemistry) of Ku70 in U-2 OS cells. Samples were incubated with Ku70 Monoclonal antibody (Product # MA5-35083) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Product Details
MA5-35083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0551
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-609 of human Ku70 (P12956)
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848988
Product Specific Information
Immunogen sequence: PEQAVDLTLP KVEAMNKRLG SLVDEFKELV YPPDYNPEGK VTKRKHDNEG SGSKRPKVEY SEEELKTHIS KGTLGKFTVP MLKEACRAYG LKSGLKKQEL LEALTKHFQD
Target Information
XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CT; CTA-216E10.7; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen protein p70 homolog; Ku autoantigen, 70kDa; Ku p70; Ku70; Lupus Ku autoantigen protein p70; OTTHUMP00000028581; thyroid autoantigen 70 kDa; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6
Gene Aliases: 70kDa; CTC75; CTCBF; G22P1; KU70; ML8; TLAA; XRCC6
UniProt ID: (Human) P12956, (Mouse) P23475
Entrez Gene ID: (Human) 2547, (Mouse) 14375
DNA binding
damaged DNA binding
double-stranded DNA binding
double-stranded telomeric DNA binding
ATP-dependent DNA helicase activity
protein binding
ATP binding
protein C-terminus binding
transcription regulatory region DNA binding
poly(A) RNA binding
macromolecular complex binding
5'-deoxyribose-5-phosphate lyase activity
nucleotide binding
catalytic activity
helicase activity
hydrolase activity
lyase activity
telomeric DNA binding
DNA helicase
telomere maintenance
DNA ligation
double-strand break repair via nonhomologous end joining
DNA recombination
transcription, DNA-templated
positive regulation of type I interferon production
DNA duplex unwinding
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of smooth muscle cell proliferation
protein heterotetramerization
cellular response to gamma radiation
cellular response to X-ray
establishment of integrated proviral latency
double-strand break repair via classical nonhomologous end joining
DNA repair
double-strand break repair
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
metabolic process
response to ionizing radiation
V(D)J recombination
positive regulation of neurogenesis
cellular hyperosmotic salinity response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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