Proteintech

LIG3 Polyclonal Antibody

LIG3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

LIG3 Antibody (26583-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 26583-1-AP (LIG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

LIG3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

26583-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LIG3 Fusion Protein Ag24998 (254-390 aa encoded by BC068005)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: CDPRHKDCL LREFRKLCAM VADNPSYNTK TQIIQDFLRK GSAGDGFHGD VYLTVKLLLP GVIKTVYNLN DKQIVKLFSR IFNCNPDDMA RDLEQGDVSE TIRVFFEQSK SFPPAAKSLL TIQEVDEFLL RLSKLTKE (254-390 aa encoded by BC068005)

Target Information

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97 kDa (beta form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy.

Brain : a journal of neurology

Bonora E,Chakrabarty S,Kellaris G,Tsutsumi M,Bianco F,Bergamini C,Ullah F,Isidori F,Liparulo I,Diquigiovanni C,Masin L,Rizzardi N,Cratere MG,Boschetti E,Papa V,Maresca A,Cenacchi G,Casadio R,Martelli P,Matera I,Ceccherini I,Fato R,Raiola G,Arrigo S,Signa S,Sementa AR,Severino M,Striano P,Fiorillo C,Goto T,Uchino S,Oyazato Y,Nakamura H,Mishra SK,Yeh YS,Kato T,Nozu K,Tanboon J,Morioka I,Nishino I,Toda T,Goto YI,Ohtake A,Kosaki K,Yamaguchi Y,Nonaka I,Iijima K,Mimaki M,Kurahashi H,Raams A,MacInnes A,Alders M,Engelen M,Linthorst G,de Koning T,den Dunnen W,Dijkstra G,van Spaendonck K,van Gent DC,Aronica EM,Picco P,Carelli V,Seri M,Katsanis N,Duijkers FAM,Taniguchi-Ikeda M,De Giorgio R

Tue Jun 22 00:00:00 EDT 2021

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

The international journal of biochemistry & cell biology

Li G,Zhang X,Wang H,Liu D,Li Z,Wu Z,Yang H

Sat Aug 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: DNA ligase (ATP) 3; DNA ligase 3; DNA ligase III; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 3

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Gene Aliases: D11Wsu78e; LIG2; LIG3

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UniProt ID: (Human) P49916

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Entrez Gene ID: (Human) 3980, (Mouse) 16882

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Function(s)

DNA binding DNA ligase activity DNA ligase (ATP) activity protein binding ATP binding zinc ion binding nucleotide binding ligase activity metal ion binding DNA ligase

Process(es)

double-strand break repair via homologous recombination lagging strand elongation transcription-coupled nucleotide-excision repair base-excision repair, DNA ligation nucleotide-excision repair, DNA gap filling double-strand break repair cell cycle V(D)J recombination cell division DNA biosynthetic process negative regulation of mitochondrial DNA replication DNA replication DNA ligation DNA repair double-strand break repair via nonhomologous end joining DNA recombination cellular response to DNA damage stimulus mitochondrion organization mitochondrial DNA repair negative regulation of DNA recombination DNA ligation involved in DNA repair double-strand break repair via alternative nonhomologous end joining

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LIG3 Polyclonal Antibody

LIG3 Antibody (26583-1-AP) in ICC/IF

Product Details

26583-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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