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LIM1 Antibody (PA5-120545) in WB
Western blot analysis of LIM1 in extracts of various cell lines. Samples were incubated with LIM1 Polyclonal antibody (Product # PA5-120545) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120545
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-180 of human LHX1 (NP_005559.2).
Conjugate
Form
Liquid
Concentration
2.82 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914117
Product Specific Information
Positive test controls include: U-87MG, Mouse testis, Mouse brain, Rat testis, Rat brain. The target is usually found in the following locations: Nucleus.
Immunogen sequence: SNSSVAKENS LHSATTGSDP SLSPDSQDPS QDDAKDSESA NVSDKEAGSN ENDDQNLGAK R
Target Information
This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hLim-1; Homeobox protein Lim-1; LHX-1; LIM homeo box protein 1; LIM homeobox protein 1; LIM/homeobox protein Lhx1; MGC126723; MGC138141; Rlim
Gene Aliases: LHX1; LIM-1; LIM1
UniProt ID: (Human) P48742, (Mouse) P63006, (Rat) P63007
Entrez Gene ID: (Human) 3975, (Mouse) 16869, (Rat) 257634
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
zinc ion binding
sequence-specific DNA binding
DNA binding
protein binding
metal ion binding
helix-turn-helix transcription factor
homeodomain transcription factor
DNA-binding transcription factor
gene-specific transcriptional regulator
urogenital system development
ureteric bud development
branching involved in ureteric bud morphogenesis
gastrulation with mouth forming second
ectoderm formation
endoderm formation
kidney development
transcription from RNA polymerase II promoter
cell-cell signaling
pattern specification process
nervous system development
motor neuron axon guidance
anatomical structure morphogenesis
post-embryonic development
embryonic pattern specification
organ morphogenesis
anterior/posterior axis specification
anterior/posterior pattern specification
dorsal/ventral pattern formation
regulation of gene expression
retina layer formation
ventral spinal cord development
spinal cord association neuron differentiation
telencephalon development
cerebellum development
cerebellar Purkinje cell differentiation
forebrain regionalization
cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation
somite rostral/caudal axis specification
metanephric part of ureteric bud development
oviduct epithelium development
uterine epithelium development
nephric duct elongation
horizontal cell localization
positive regulation of embryonic development
cellular response to fibroblast growth factor stimulus
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
anatomical structure formation involved in morphogenesis
embryonic viscerocranium morphogenesis
pronephros development
embryonic retina morphogenesis in camera-type eye
uterus development
oviduct development
cervix development
vagina development
head development
epithelium development
paramesonephric duct development
comma-shaped body morphogenesis
S-shaped body morphogenesis
renal vesicle morphogenesis
mesonephric duct development
nephric duct morphogenesis
metanephric glomerulus development
metanephric comma-shaped body morphogenesis
metanephric renal vesicle morphogenesis
metanephric S-shaped body morphogenesis
primitive streak formation
positive regulation of branching involved in ureteric bud morphogenesis
lateral motor column neuron migration
positive regulation of gastrulation
positive regulation of anterior head development
positive regulation of nephron tubule epithelial cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
renal system development
mesonephric tubule development
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