Invitrogen

IL-4 Monoclonal Antibody (25D2), Biotin

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Datasheet
Protocols
Questions & Answers

Product Details

M450B

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

ELISA (ELISA)

0.003-12.5 µg/mL
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

25D2

Immunogen

Recombinant human IL-4 (CHO cell-derived)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223569

Product Specific Information

M450B targets IL-4 in WB and ELISA applications and shows reactivity with Human samples.

The M450B immunogen is recombinant human IL-4 (CHO cell-derived).

M450B detects IL-4 which has a predicted molecular weight of approximately 15 kDa.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)
cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily
Process(es)
microglial cell activation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment negative regulation of acute inflammatory response negative regulation of chronic inflammatory response chemotaxis immune response cellular defense response female pregnancy response to nutrient cholesterol metabolic process regulation of proton transport negative regulation of epithelial cell migration response to organic cyclic compound B cell differentiation positive regulation of B cell proliferation B cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-13 production response to cytokine T-helper 2 cell cytokine production type 2 immune response positive regulation of T cell proliferation positive regulation of activated T cell proliferation regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan myeloid dendritic cell differentiation negative regulation of macrophage activation negative regulation of apoptotic process positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation positive regulation of chemokine biosynthetic process connective tissue growth factor biosynthetic process regulation of isotype switching positive regulation of MHC class II biosynthetic process response to ethanol positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes regulation of immune response positive regulation of sequence-specific DNA binding transcription factor activity retina development in camera-type eye negative regulation of white fat cell proliferation cellular response to mercury ion positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of myoblast fusion negative regulation of complement-dependent cytotoxicity negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis negative regulation of extrinsic apoptotic signaling pathway
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