Invitrogen

EpCAM Monoclonal Antibody (VU-1D9), PE

FIGURE: 1 / 1

EpCAM Antibody (MA1-10198) in Flow

Flow cytometry analysis (surface staining) of human MCF-7 and SP2 cell lines with anti-human CD326 / EpCAM (VU-1D9) PE Monoclonal antibody (Product # MA1-10198).

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Product Details

MA1-10198

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

20 µL/1x10^6 cells

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VU-1D9

Immunogen

Small cell lung carcinoma cell line H69.

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154356

Product Specific Information

This antibody recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells.

Bioactive materials

Shen H,Su R,Peng J,Zhu L,Deng K,Niu Q,Song Y,Yang L,Wu L,Zhu Z,Yang C

MA1-10198 was used in Flow cytometry/Cell sorting to establish an antibody-engineered red blood cell (RBC-Ab) affinity interface on microfluidic chip for high-performance isolation and release of CTCs.

Sun May 01 00:00:00 EDT 2022

CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.

EBioMedicine

Sun Y,Wu G,Cheng KS,Chen A,Neoh KH,Chen S,Tang Z,Lee PF,Dai M,Han RPS

MA1-10198 was used in Flow cytometry to report on the clinical value of a phenotypic circulating tumor cell (CTC)-based blood test for a preoperative prognostic assessment of tumor metastasis and overall survival (OS) of PDAC patients.

Thu Aug 01 00:00:00 EDT 2019

New frontiers in circulating tumor cell analysis: A reference guide for biomolecular profiling toward translational clinical use.

International journal of cancer

Becker TM,Caixeiro NJ,Lim SH,Tognela A,Kienzle N,Scott KF,Spring KJ,de Souza P

MA1-10198 was used in immunocytochemistry to review the current state-of-the-art in circulating tumor cell profiling

Sun Jun 01 00:00:00 EDT 2014

Systematic analysis and validation of differential gene expression in ovarian serous adenocarcinomas and normal ovary.

Journal of cancer research and clinical oncology

Bauerschlag D,Bräutigam K,Moll R,Sehouli J,Mustea A,Salehin D,Krajewska M,Reed JC,Maass N,Hampton GM,Meinhold-Heerlein I

MA1-10198 was used in immunohistochemistry to identify genes differentially expressed in ovaraian serous adenocarcinoma as compared to healthy ovarian tissue

Fri Feb 01 00:00:00 EST 2013

Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

PloS one

Mosoyan G,Nagi C,Marukian S,Teixeira A,Simonian A,Resnick-Silverman L,DiFeo A,Johnston D,Reynolds SR,Roses DF,Mosoian A

MA1-10198 was used in flow cytometry to study the different molecular phenotype and tumorigenicity of 5 breast cancer cell lines derived from a single patient

Fri Jul 26 00:00:00 EDT 2013

Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.

Nature genetics

Ligtenberg MJ,Kuiper RP,Chan TL,Goossens M,Hebeda KM,Voorendt M,Lee TY,Bodmer D,Hoenselaar E,Hendriks-Cornelissen SJ,Tsui WY,Kong CK,Brunner HG,van Kessel AG,Yuen ST,van Krieken JH,Leung SY,Hoogerbrugge N

MA1-10198 was used in immunohistochemistry to investigate Lynch syndrome with MSH2-deficient tumors

Thu Jan 01 00:00:00 EST 2009

Gene expression patterns in ovarian carcinomas.

Molecular biology of the cell

Schaner ME,Ross DT,Ciaravino G,Sorlie T,Troyanskaya O,Diehn M,Wang YC,Duran GE,Sikic TL,Caldeira S,Skomedal H,Tu IP,Hernandez-Boussard T,Johnson SW,O'Dwyer PJ,Fero MJ,Kristensen GB,Borresen-Dale AL,Hastie T,Tibshirani R,van de Rijn M,Teng NN,Longacre TA,Botstein D,Brown PO,Sikic BI

MA1-10198 was used in immunohistochemistry to study gene expression patterns in ovarian carcinomas using DNA microarrays

Sat Nov 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Tumor-associated calcium signal transducer 1

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Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

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UniProt ID: (Human) P16422

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Entrez Gene ID: (Human) 4072

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Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EpCAM Monoclonal Antibody (VU-1D9), PE

EpCAM Antibody (MA1-10198) in Flow

Product Details

MA1-10198

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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