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MAGE1 Antibody (MA5-37821) in WB
Western blot analysis of MAGE1 in extracts of various cell lines. Samples were incubated with MAGE1 Monoclonal antibody (Product # MA5-37821) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Product Details
MA5-37821
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1839
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-309 of human MAGE-1/MAGE1A (P43355)
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897744
Product Specific Information
Positive Samples: HeLa, A-549, A375, Mouse lung, Mouse testis
Immunogen sequence: VMIAMEGGHA PEEEIWEELS VMEVYDGREH SAYGEPRKLL TQDLVQEKYL EYRQVPDSDP ARYEFLWGPR ALAETSYVKV LEYVIKVSAR VRFFFPSLRE AALREEEEGV
Target Information
Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). An important group of antigens is coded by the MAGE family of genes. It was identified that melanoma associated with antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various hisological tyoes (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen MZ2-E; Cancer/testis antigen 1.1; cancer/testis antigen family 1, member 1; CT1.1; mage 1; mage-1; MAGE-1 antigen; melanoma antigen family A 1; melanoma antigen family A, 1; melanoma antigen family A, 1 (directs expression of antigen MZ2-E); melanoma antigen family A1; melanoma antigen MAGE-1; Melanoma-associated antigen 1; melanoma-associated antigen MZ2-E
Gene Aliases: CT1.1; Mage-a1; MAGE1; MAGE1A; MAGEA1
UniProt ID: (Human) P43355
Entrez Gene ID: (Human) 4100, (Mouse) 17137
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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