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MAGEH1 Antibody (PA5-99777) in ICC/IF
Immunocytochemistry analysis of MAGEH1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MAGEH1 polyclonal antibody (Product # PA5-99777) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99777
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAGEH1(Accession Q9H213), corresponding to amino acid residues M1-S42.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818710
Product Specific Information
Antibody detects endogenous levels of total MAGH1.
Target Information
Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGEH1 is not fully elucidated, it may play a role in embryonal development and tumor transformation or aspects of tumor progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-Apr; Apoptosis-related protein 1; APR-1; MAGE-H1 antigen; melanoma antigen family H, 1; melanoma antigen family H1; Melanoma-associated antigen H1; Restin
Gene Aliases: 2010107K23Rik; APR-1; APR1; MAGEH; MAGEH1
UniProt ID: (Human) Q9H213, (Mouse) Q9NWG9, (Rat) Q5PPP4
Entrez Gene ID: (Human) 28986, (Mouse) 75625, (Rat) 367767
Performance Guarantee
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