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MAPK15 Antibody (PA5-105740) in ICC/IF
Immunofluorescent analysis of MAPK15 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MAPK15 polyclonal antibody (Product # PA5-105740) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-105740
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAPK15(Accession Q8TD08), corresponding to amino acid residues V151-M201.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853923
Product Specific Information
Antibody detects endogenous levels of total ERK8.
Target Information
The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.11.24; ERK-7; ERK-8; extracellular regulated kinase 8 delta; extracellular signal regulated kinase 8; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8; MAP kinase 15; MAPK 15; Mitogen-activated protein kinase 15
Gene Aliases: BC048082; ERK7; ERK8; MAPK15
UniProt ID: (Human) Q8TD08, (Mouse) Q80Y86
Entrez Gene ID: (Human) 225689, (Mouse) 332110
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
positive regulation of protein phosphorylation
negative regulation of DNA replication
positive regulation of protein ubiquitination
positive regulation of telomere maintenance via telomerase
response to estradiol
positive regulation of protein catabolic process
protein autophosphorylation
positive regulation of telomerase activity
positive regulation of telomere capping
protein phosphorylation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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