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MBTPS1 Antibody (PA5-109948) in ICC/IF
Immunofluorescence analysis of Membrane-bound transcription factor site-1 protease was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MBTPS1 Polyclonal Antibody (Product # PA5-109948) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibod... View More
Product Details
PA5-109948
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 803-1052 of human MBTPS1 (NP_003782.1).
Conjugate
Form
Liquid
Concentration
1.73 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855359
Product Specific Information
Immunogen sequence: ITQTFKDQGL EVLKQETAVV ENVPILGLYQ IPAEGGGRIV LYGDSNCLDD SHRQKDCFWL LDALLQYTSY GVTPPSLSHS GNRQRPPSGA GSVTPERMEG NHLHRYSKVL EAHLGDPKPR PLPACPRLSW AKPQPLNETA PSNLWKHQKL LSIDLDKVVL PNFRSNRPQV RPLSPGESGA WDIPGGIMPG RYNQEVGQTI PVFAFLGAMV VLAFFVVQIN KAKSRPKRRK PRVKRPQLMQ QVHPPKTPSV
Target Information
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the cis/medial-Golgi where a second autocatalytic event takes place and the catalytic activity is acquired. It encodes a type 1 membrane bound protease which is ubiquitously expressed and regulates cholesterol or lipid homeostasis via cleavage of substrates at non-basic residues. Mutations in this gene may be associated with lysosomal dysfunction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endopeptidase S1P; membrane-bound transcription factor peptidase, site 1; membrane-bound transcription factor protease, site 1; Membrane-bound transcription factor site-1 protease; proprotein convertase subtilisin/kexin type 8; S1P endopeptidase; site-1 protease; SKI-1; Sterol-regulated luminal protease; subtilisin kexin isozyme 1; Subtilisin/kexin isozyme 1; subtilisin/kexin isozyme SKI-1; subtilisin/kexin isozyme-1; Subtilisin/kexin-isozyme 1
Gene Aliases: 0610038M03Rik; AV003995; KIAA0091; MBTPS1; mKIAA0091; PCSK8; S1P; SKI-1; SKI1
UniProt ID: (Human) Q14703, (Mouse) Q9WTZ2
Entrez Gene ID: (Human) 8720, (Mouse) 56453
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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