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CD4 Monoclonal Antibody (RM4-5), PE-Texas Red

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Published Figures (8)

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CD4 Antibody (MCD0417) in Flow

Oncoimmunology 2020 - Figure 3. Effect of anti-CD20 + no-alpha mutein combination therapy on CD4 + T cell subsets. The anti-CD20 + no-alpha mutein combination therapy increased the percentages of Th1/(IFNgamma + CD4 + CD3 + ) (a), kept the percentages of Th17/(IL-17A + CD4 + CD3 + ) to the level of anti-CD20 mAb-treated mice (b) and induced a decrease of the percentage of Treg/(FoxP3 + CD4 + CD3 + ), (c) of stimulated splenocyte... View More

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Product Details

MCD0417

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Immunogen

Mouse CD4

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

Excitation/Emission Max

567/615 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS with BSA, sucrose

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373812

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cyto-Immuno-Therapy for Cancer: A Pathway Elicited by Tumor-Targeted, Cytotoxic Drug-Packaged Bacterially Derived Nanocells.

Cancer cell

Sagnella SM,Yang L,Stubbs GE,Boslem E,Martino-Echarri E,Smolarczyk K,Pattison SL,Vanegas N,St Clair E,Clarke S,Boockvar J,MacDiarmid JA,Brahmbhatt H

MCD0417 was used in Flow cytometry/Cell sorting to demonstrate that tumor-targeting EDV nanocells function as an immunotherapeutic by delivering a cytotoxin in conjunction with activation of the immune system.

Mon Mar 16 00:00:00 EDT 2020

A rationally-engineered IL-2 improves the antitumor effect of anti-CD20 therapy.

Oncoimmunology

Casadesús AV,Deligne C,Diallo BK,Sosa K,Josseaume N,Mesa C,León K,Hernández T,Teillaud JL

MCD0417 was used in Flow cytometry/Cell sorting to show that the combination of anti-tumor antibodies and no-alpha mutein is a promising approach to improve the therapeutic effect of these antibodies by potentiating NK/macrophage-mediated innate immunity and the adaptive T-cell response.

Tue Jun 02 00:00:00 EDT 2020

A rationally-engineered IL-2 improves the antitumor effect of anti-CD20 therapy.

Oncoimmunology

Casadesús AV,Deligne C,Diallo BK,Sosa K,Josseaume N,Mesa C,León K,Hernández T,Teillaud JL

Tue Jun 02 00:00:00 EDT 2020

The deacetylase Sirt1 is an essential regulator of Aire-mediated induction of central immunological tolerance.

Nature immunology

Chuprin A,Avin A,Goldfarb Y,Herzig Y,Levi B,Jacob A,Sela A,Katz S,Grossman M,Guyon C,Rathaus M,Cohen HY,Sagi I,Giraud M,McBurney MW,Husebye ES,Abramson J

MCD0417 was used in flow cytometry to determine the role of Sirt1 in regulating the expression of tissue-restricted antigens in medullary thymic epithelial cells.

Wed Jul 01 00:00:00 EDT 2015

Histamine H₂ receptor signaling × environment interactions determine susceptibility to experimental allergic encephalomyelitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Saligrama N,Case LK,Krementsov DN,Teuscher C

MCD0417 was used in flow cytometry to demonstrate that regulation of encephalitogenic T-cell responses and EAE susceptibility by H2R signaling in CD4(+) T cells is dependent on genetic and environmental interactions.

Tue Apr 01 00:00:00 EDT 2014

Histamine H₂ receptor signaling × environment interactions determine susceptibility to experimental allergic encephalomyelitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Saligrama N,Case LK,Krementsov DN,Teuscher C

Tue Apr 01 00:00:00 EDT 2014

Systemic lack of canonical histamine receptor signaling results in increased resistance to autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Saligrama N,Case LK,del Rio R,Noubade R,Teuscher C

MCD0417 was used in flow cytometry to investigate the contribution of histamine on the development of experimental allergic encephalomyelitis.

Mon Jul 15 00:00:00 EDT 2013

The neonatal CNS is not conducive for encephalitogenic Th1 T cells and B cells during experimental autoimmune encephalomyelitis.

Journal of neuroinflammation

Cravens PD,Kieseier BC,Hussain R,Herndon E,Arellano B,Ben LH,Timmons BC,Castro-Rojas C,Hartung HP,Hemmer B,Weber MS,Zamvil SS,Stüve O

MCD0417 was used in flow cytometry to test if a differential composition of immune cells within the central nervous system modulates the age-associated susceptibility to autoimmune disease.

Fri May 24 00:00:00 EDT 2013

Histamine H(3) receptor integrates peripheral inflammatory signals in the neurogenic control of immune responses and autoimmune disease susceptibility.

PloS one

Krementsov DN,Wall EH,Martin RA,Subramanian M,Noubade R,Del Rio R,Mawe GM,Bond JP,Poynter ME,Blankenhorn EP,Teuscher C

MCD0417 was used in flow cytometry to characterize Hrh3 polymorphisms between experimental allergic encephalomyelitis-susceptible and resistant mice.

Mon Feb 24 00:00:00 EST 2014

Combinatorial roles for histamine H1-H2 and H3-H4 receptors in autoimmune inflammatory disease of the central nervous system.

European journal of immunology

Saligrama N,Noubade R,Case LK,del Rio R,Teuscher C

MCD0417 was used in flow cytometry to test if combinatorial targeting of histamine receptors is an effective therapy for experimental allergic encephalomyelitis.

Fri Jun 01 00:00:00 EDT 2012

Histamine H4 receptor optimizes T regulatory cell frequency and facilitates anti-inflammatory responses within the central nervous system.

Journal of immunology (Baltimore, Md. : 1950)

del Rio R,Noubade R,Saligrama N,Wall EH,Krementsov DN,Poynter ME,Zachary JF,Thurmond RL,Teuscher C

Published figure using CD4 monoclonal antibody (Product # MCD0417) in Flow Cytometry

Sun Jan 15 00:00:00 EST 2012

Genetics of experimental allergic encephalomyelitis supports the role of T helper cells in multiple sclerosis pathogenesis.

Annals of neurology

Blankenhorn EP,Butterfield R,Case LK,Wall EH,del Rio R,Diehl SA,Krementsov DN,Saligrama N,Teuscher C

MCD0417 was used in flow cytometry to identify genes involved in multiple sclerosis and experimental allergic encephalomyelitis.

Thu Dec 01 00:00:00 EST 2011

ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection.

Journal of neuroimmunology

Tse MC,Lane C,Mott K,Onlamoon N,Hsiao HM,Perng GC

MCD0417 was used in Flow cytometry/Cell sorting to suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.

Tue Aug 18 00:00:00 EDT 2009

Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation.

Blood

Chen BJ,Cui X,Sempowski GD,Domen J,Chao NJ

MCD0417 was used in flow cytometry to test if higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation mouse model

Tue Jun 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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Gene Aliases: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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Function(s)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

Process(es)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (RM4-5), PE-Texas Red

CD4 Antibody (MCD0417) in Flow

Product Details

MCD0417

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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