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FIGURE: 1 / 1
MCF2 Antibody (PA5-88796) in WB
Western blot analysis of MCF2 in extracts of HT-29 cells. Samples were incubated with MCF2 Polyclonal antibody (Product # PA5-88796) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88796
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 872-1001 of human MCF2 (NP_0011653471)
Conjugate
Form
Liquid
Concentration
0.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805138
Product Specific Information
Immunogen sequence: VKMTWLKEIR NILLKQQELL TVKKRKQQDQ LTERDKFQIS LQQNDEKQQG AFISTEETEL EHTSTVVEVC EAIASVQAEA NTVWTEASQS AEISEEPAEW SSNYFYPTYD ENEEENRPLM RPVSEMALLY; Positive Samples: HT-29; Cellular Location: Cytoplasm, Membrane
Target Information
Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Oncogene MCF2 (oncogene DBL); OTTHUMP00000024159; Proto-oncogene DBL; Proto-oncogene MCF-2
Gene Aliases: ARHGEF21; B230117G22Rik; DBL; Mcf-2; MCF2
UniProt ID: (Human) P10911
Entrez Gene ID: (Human) 4168, (Mouse) 109904
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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