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Product Details
PA5-120637
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 374-465 of human MKNK1 (NP_003675.2).
Conjugate
Form
Liquid
Concentration
0.829 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914209
Product Specific Information
Positive test controls include: HeLa, 293T, HepG2, Mouse pancreas, Rat skeletal muscle. The target is usually found in the following locations: Cytoplasm, Cytoplasm, Nucleus.
Immunogen sequence: VQGQAPEKGL PTPQVLQRNS STMDLTLFAA EAIALNRQLS QHEENELAEE PEALADGLCS MKLSPPCKSR LARRRALAQA GRGEDRSPPT AL
Target Information
The Map kinase-interacting kinases (MKNK1 and MKNK2) are activated by Map kinases, regulate protein synthesis by phosphroylation eIF4E on Ser209, and may contribute to tumorigenesis. Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal-integrating kinase 1; mnk1; OTTHUMP00000009675; OTTHUMP00000009676
Gene Aliases: 2410048M24Rik; MKNK1; MNK1
UniProt ID: (Human) Q9BUB5, (Mouse) O08605, (Rat) Q4G050
Entrez Gene ID: (Human) 8569, (Mouse) 17346, (Rat) 500526
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
calcium-dependent protein serine/threonine kinase activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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