Invitrogen

MyD88 Polyclonal Antibody, eBioscience™

3 Published Figures
5 References
View all (38) MyD88 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-6223-63

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
View 5 publications 5 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468046

Product Specific Information

Description: The polyclonal antibody reacts with human and mouse MyD88; the antibody was raised against residues 279-296 of human MyD88 and recognizes human and mouse antigens. MyD88, a myeloid differentiation primary response gene, is expressed in a variety of tissues and functions as an adapter molecule in the IL-1 signaling pathway involved in the inflammatory responses induced by cytokines and LPS. MyD88 associates with and recruits IRAK to the IL-1 receptor. Dominant negative mutants of MyD88 attenuate IL-1R-mediated NF-kappaB activation. MyD88 also functions as a regulator molecule for IL-18 receptor and human Toll receptor family. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to LPS.

Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested: This polyclonal antibody has been reported for use in immunoblotting (1:500-1:1000 dilution) of MyD88 from Jurkat cell lysate as a positive control. A 35 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: mutant myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); Myeloid differentiation primary response protein MyD88

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Gene Aliases: MYD88; MYD88D

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UniProt ID: (Human) Q99836, (Mouse) P22366

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Entrez Gene ID: (Human) 4615, (Mouse) 17874

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Function(s)
death receptor binding protein binding identical protein binding protein self-association TIR domain binding receptor binding Toll binding interleukin-1 receptor binding scaffold/adaptor protein
Process(es)
toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway apoptotic process inflammatory response signal transduction cell surface receptor signaling pathway positive regulation of type I interferon production positive regulation of interleukin-17 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production toll-like receptor 9 signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity response to interleukin-1 3'-UTR-mediated mRNA stabilization cellular response to mechanical stimulus response to molecule of fungal origin immune system process immune response transmembrane receptor protein serine/threonine kinase signaling pathway JNK cascade Toll signaling pathway response to virus immunoglobulin mediated immune response cytokine-mediated signaling pathway lipopolysaccharide-mediated signaling pathway response to peptidoglycan response to lipopolysaccharide positive regulation of tumor necrosis factor production regulation of cell proliferation negative regulation of growth of symbiont in host positive regulation of chemokine biosynthetic process type I interferon biosynthetic process positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation establishment of endothelial intestinal barrier
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