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MyD88 Antibody (PA5-19918) in ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling MyD88 with MyD88 Polyclonal Antibody (Product # PA5-19918) at 10 µg/mL, followed by Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
Product Details
PA5-19918
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 16 amino acid peptide near the center of human MYD88 isoform 1
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152014
Product Specific Information
A suggested positive control is Jurkat cell lysate.
PA5-19918 can be used with blocking peptide PEP-0044.
The PA5-19918 The immunogen is located within amino acids 220 - 270 of MYD88.
Predicted molecular ~ 35kD. In Western blot applications, this antibody has been observed to detect a band at: 35kD
Predicted species reactivity based on immunogen sequence: Pig: (100%), Sheep: (100%), Bovine: (100%), Chicken: (100%), Rat: (88%), Mouse (88%)
Target Information
Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: mutant myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); myeloid differentiation primary response gene 88; Myeloid differentiation primary response protein MyD88
Gene Aliases: MYD88; MYD88D
UniProt ID: (Human) Q99836, (Rat) Q6Y1S1, (Mouse) P22366
Entrez Gene ID: (Human) 4615, (Rat) 301059, (Mouse) 17874
toll-like receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
apoptotic process
inflammatory response
signal transduction
cell surface receptor signaling pathway
positive regulation of type I interferon production
positive regulation of interleukin-17 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
toll-like receptor 9 signaling pathway
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
response to interleukin-1
3'-UTR-mediated mRNA stabilization
cellular response to mechanical stimulus
response to molecule of fungal origin
JNK cascade
Toll signaling pathway
response to virus
immunoglobulin mediated immune response
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
response to peptidoglycan
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
regulation of cell proliferation
negative regulation of growth of symbiont in host
positive regulation of chemokine biosynthetic process
type I interferon biosynthetic process
positive regulation of JNK cascade
positive regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
establishment of endothelial intestinal barrier
immune system process
immune response
transmembrane receptor protein serine/threonine kinase signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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