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MyD88 Antibody (MA5-55295) in WB
Western Blot analysis of various lysates using MyD88 Recombinant Monoclonal Antibody (Product # MA5-55295) at 1:8,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Product Details
MA5-55295
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0J1O5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MyD88.
Conjugate
Form
Liquid
Concentration
0.61 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: LAEEMDFEYL EIRQLETQAD PTGRLLDAWQ GRPGASVGRL LELLTKLGRD DVLLELGPSI EEDCQKYILK QQQEEAEKPL QVAAVDSSVP RTAELAGITT L
Target Information
Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mutant myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); myeloid differentiation primary response gene 88; Myeloid differentiation primary response protein MyD88
Gene Aliases: MYD88; MYD88D
UniProt ID: (Human) Q99836, (Rat) Q6Y1S1, (Mouse) P22366
Entrez Gene ID: (Human) 4615, (Rat) 301059, (Mouse) 17874
toll-like receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
apoptotic process
inflammatory response
signal transduction
cell surface receptor signaling pathway
positive regulation of type I interferon production
positive regulation of interleukin-17 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
toll-like receptor 9 signaling pathway
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
response to interleukin-1
3'-UTR-mediated mRNA stabilization
cellular response to mechanical stimulus
response to molecule of fungal origin
JNK cascade
Toll signaling pathway
response to virus
immunoglobulin mediated immune response
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
response to peptidoglycan
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
regulation of cell proliferation
negative regulation of growth of symbiont in host
positive regulation of chemokine biosynthetic process
type I interferon biosynthetic process
positive regulation of JNK cascade
positive regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
establishment of endothelial intestinal barrier
immune system process
immune response
transmembrane receptor protein serine/threonine kinase signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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