Invitrogen

MyD88 Monoclonal Antibody (4D6)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (38) MyD88 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MyD88 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

MyD88 Antibody (MA5-16231) in ICC/IF

Immunocytochemistry analysis of MyD88 in MCF-7 cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 5 µg/mL. Isotype control (top) and MyD88 Antibody (bottom).

Product Image Gallery

MyD88 Antibody — Figure 5 Myeloid differentiation primary response gene 88 (MyD88) is the adaptor protein for toll-like receptor (TLR)-10 signaling following stimulation by dsRNA. (A) Alignment of toll/interleukin-1 receptor domain sequences of human TLRs. Sequence logo (top) represents the conserved motif identified by MEME. Sequence in black box is the BB-loop sequence in TLR10. The alanine/proline residues highlighted in green determine the adaptor protein bound by TLRs. All human TLRs, except TLR3, have proline in the BB-loop. (B) Confocal micrograph of THP-1 cells stimulated with 10 ug/ml poly(I:C) stained at different time points for TLR10 (red), MyD88 (green), with nuclei stained with DNA-binding dye 4',6-Diamidin-2-phenylindol (DAPI) (blue). Unstimulated (US) cells were included as a control. Arrows indicate the co-localization of TLR10 and MyD88 (yellow). Inset (at 10 min post-challenge) is an enlargement of the white square box. Scale bars, 5 um. (C) TLR10 interacts with MyD88 upon poly(I:C) stimulation. Cell lysates of THP-1 cells stimulated with 10 ug/ml poly(I:C) at different time points were immunoprecipitated using anti-TLR10 antibody and then analyzed by Western blotting using anti-MyD88 or anti-TRIF antibodies. beta-ACTIN was the input control. Data shown are representative of at least two independent experiments. {TM}

Product Details

MA5-16231

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

0.5 µg per million cells

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4D6

Immunogen

A portion of amino acids 50-100 of human MyD88

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537749

Target Information

Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recognition of Double-Stranded RNA and Regulation of Interferon Pathway by Toll-Like Receptor 10.

Frontiers in immunology

Lee SM,Yip TF,Yan S,Jin DY,Wei HL,Guo RT,Peiris JSM

Published figure using MyD88 monoclonal antibody (Product # MA5-16231) in Western Blot

Fri May 10 00:00:00 EDT 2019

Association between toll-like receptor 8 expression and adverse clinical outcomes in patients with enterovirus-associated dilated cardiomyopathy.

American heart journal

Satoh M,Akatsu T,Ishikawa Y,Minami Y,Takahashi Y,Nakamura M

MA5-16231 was used in immunohistochemistry to study the prognostic significance of TLR8 expression in patients with enterovirus-associated dilated cardiomyopathy

Sat Sep 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: mutant myeloid differentiation primary response 88; myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein 88; Myeloid differentiation primary response protein MyD88

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Gene Aliases: MYD88; MYD88D

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UniProt ID: (Human) Q99836, (Rhesus monkey) B3Y683, (Mouse) P22366

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Entrez Gene ID: (Human) 4615, (Rhesus monkey) 696494, (Mouse) 17874

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Function(s)

death receptor binding protein binding identical protein binding protein self-association TIR domain binding receptor binding Toll binding interleukin-1 receptor binding scaffold/adaptor protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MyD88 Monoclonal Antibody (4D6)

MyD88 Antibody (MA5-16231) in ICC/IF

Product Details

MA5-16231

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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