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MyD88 Antibody (MA5-51249) in WB
Western blot analysis of MyD88 in extracts of Rat lung. Samples were incubated with MyD88 Recombinant monoclonal antibody (Product # MA5-51249) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
MA5-51249
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5R9C5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MyD88 (NP_002459.3).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093901
Product Specific Information
Immunogen sequence: LAEEMDFEYL EIRQLETQAD PTGRLLDAWQ GRPGASVGRL LELLTKLGRD DVLLELGPSI EEDCQKYILK QQQEEAEKPL QVAAVDSSVP RTAELAGITT L
Target Information
Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: myeloid differentiation primary response gene 88; Myeloid differentiation primary response protein MyD88
Gene Aliases: Myd88
UniProt ID: (Rat) Q6Y1S1, (Mouse) P22366
Entrez Gene ID: (Rat) 301059, (Mouse) 17874
response to molecule of fungal origin
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
cell surface receptor signaling pathway
JNK cascade
Toll signaling pathway
response to virus
immunoglobulin mediated immune response
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
response to peptidoglycan
response to lipopolysaccharide
positive regulation of interleukin-17 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
regulation of cell proliferation
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of growth of symbiont in host
positive regulation of chemokine biosynthetic process
innate immune response
type I interferon biosynthetic process
positive regulation of JNK cascade
positive regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
response to interleukin-1
3'-UTR-mediated mRNA stabilization
cellular response to mechanical stimulus
establishment of endothelial intestinal barrier
immune system process
immune response
signal transduction
transmembrane receptor protein serine/threonine kinase signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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