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FIGURE: 1 / 1
NAC1 Antibody (PA5-88235) in WB
Western blot analysis of NAC1 in extracts of various cell lines. Samples were incubated with NAC1 Polyclonal antibody (Product # PA5-88235) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88235
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 170-340 of human NACC1 (NP_4431081)
Conjugate
Form
Liquid
Concentration
0.84 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804762
Product Specific Information
Immunogen sequence: QQESDSVQCM PVAKRLWDSG QKEAGGGGNG SRKMAKFSTP DLAANRPHQP PPPQQAPVVA AAQPAVAAGA GQPAGGVAAA GGVVSGPSTS ERTSPGTSSA YTSDSPGSYH NEEDEEEDGG EEGMDEQYRQ ICNMYTMYSM MNVGQTAEKV EALPEQVAPE SRNRIRVRQD L; Positive Samples: SKOV3, Hela, Jurkat, Mouse brain, Mouse kidney; Cellular Location: Cytoplasm, Nucleus
Target Information
Members of the BTB/POZ family of transcriptional regulators, including BTBD14B, contain a conserved motif in the N-terminal region critical for protein-protein interactions and assembly of high molecular mass complexes (Korutla et al., 2002 [PubMed 11906783]).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BEN domain containing 8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; FLJ37383; NAC-1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; Nucleus accumbens-associated protein 1; transcriptional repressor NAC1
Gene Aliases: 2010001H03Rik; 4930511N13Rik; BEND8; BTBD14B; BTBD30; NAC-1; NAC1; NACC1
UniProt ID: (Human) Q96RE7, (Mouse) Q7TSZ8
Entrez Gene ID: (Human) 112939, (Mouse) 66830
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of transcription, DNA-templated
protein homooligomerization
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